Majamaa K, Myllylä R, Alitalo K, Vaheri A
Biochem J. 1982 Sep 15;206(3):499-503. doi: 10.1042/bj2060499.
Prolyl 3-hydroxylase activity and the extent of collagen proline 3-hydroxylation were studied in six transformed and three control human cell lines. In the transformed cell lines, the enzyme activity was markedly high in two, similar to that in control cells in two and significantly low in two. The extent of proline 3-hydroxylation was markedly high in cell lines with high enzyme activity, but it was also significantly high in some transformed cell lines with enzyme activities similar to those in the controls. The results thus suggest that, in addition to the amount of enzyme activity present, the rate of collagen synthesis also affects the extent of proline 3-hydroxylation in the newly synthesized collagen. The effect of acute cell transformation on prolyl 3-hydroxylase and 4-hydroxylase activities was studied by infecting chick-embryo fibroblasts with Rous sarcoma virus mutant NY68, temperature-sensitive for transformation. At the permissive temperature prolyl 3-hydroxylase activity showed a more rapid increase and decrease than did prolyl 4-hydroxylase activity, the maximal activity for both enzymes being about 2.5 times that in the control chick fibroblasts. When the transformed cells were shifted to the non-permissive temperature the decays in the elevated enzyme activities were similar, suggesting identical half-lives.
在六种转化的和三种对照人细胞系中研究了脯氨酰3 - 羟化酶活性以及胶原脯氨酸3 - 羟化的程度。在转化的细胞系中，两种细胞系的酶活性显著高，两种与对照细胞中的相似，两种则显著低。脯氨酸3 - 羟化程度在酶活性高的细胞系中显著高，但在一些酶活性与对照相似的转化细胞系中也显著高。因此结果表明，除了存在的酶活性量外，胶原合成速率也影响新合成胶原中脯氨酸3 - 羟化的程度。通过用对转化温度敏感的劳斯肉瘤病毒突变体NY68感染鸡胚成纤维细胞，研究了急性细胞转化对脯氨酰3 - 羟化酶和4 - 羟化酶活性的影响。在允许温度下，脯氨酰3 - 羟化酶活性比脯氨酰4 - 羟化酶活性显示出更快的升高和降低，两种酶的最大活性约为对照鸡成纤维细胞中的2.5倍。当将转化细胞转移到非允许温度时，升高的酶活性的衰减相似，表明半衰期相同。
