Lebo R V
Cytometry. 1982 Nov;3(3):145-54. doi: 10.1002/cyto.990030302.
This article focuses on current techniques and possible future developments in chromosome isolation and sorting, and DNA analysis of sorted chromosomes. The strategy of subchromosomal gene mapping by chromosome sorting is outlined and a list of cell lines containing translocated chromosomes is provided which may be used to map genes to a single chromosome with a standard fluorescence activated cell sorter. The usefulness of this and other gene mapping methods for localizing unique DNA sequences and characterizing recombinant DNA libraries constructed from sorted chromosomal DNA is also discussed.
本文重点关注染色体分离与分选的当前技术以及未来可能的发展，还有分选染色体的DNA分析。概述了通过染色体分选进行亚染色体基因定位的策略，并提供了一份含有易位染色体的细胞系清单，这些细胞系可用于通过标准的荧光激活细胞分选仪将基因定位到单条染色体上。还讨论了这种以及其他基因定位方法在定位独特DNA序列和表征由分选染色体DNA构建的重组DNA文库方面的用途。
