Ivashkin V T, Roshchin V V, Dorofeev G I, Pirumov P A, Vasil'ev V Iu
Fiziol Zh SSSR Im I M Sechenova. 1982 Oct;68(10):1410-5.
Interrelationship between the adenylate cyclase system state and utilization of succinate as a substrate of oxidation in mechanisms of HCl secretion by isolated gastric mucosa of frog Rana esculenta was studied. The inhibition of the protein synthesis by cycloheximide rendered gastric mucosa insensitive to the activation of HCl secretion by histamine and succinate. A similar effect was exerted by cimetidine, a blocking agent for H2-receptors. The blockade could be prevented with dibutyryl-cAMP. The seasonal dependence of activation of HCl secretion by specific and unspecific modifiers was discovered. The data obtained show the necessity of preliminary activation of adenylate cyclase and accumulation of limiting content of cAMP for the utilization of succinate in reactions of generation and transport of H+ by acid-producing cells of isolated gastric mucosa in frog.
研究了食用蛙离体胃黏膜盐酸分泌机制中腺苷酸环化酶系统状态与琥珀酸作为氧化底物利用之间的相互关系。放线菌酮对蛋白质合成的抑制使胃黏膜对组胺和琥珀酸激活盐酸分泌不敏感。H2受体阻断剂西咪替丁也有类似作用。二丁酰环磷腺苷可预防这种阻断作用。发现了特异性和非特异性调节剂激活盐酸分泌的季节依赖性。所获数据表明，蛙离体胃黏膜产酸细胞在利用琥珀酸进行H+生成和转运反应时，需要预先激活腺苷酸环化酶并积累有限量的环磷腺苷。
