Hennecke H, Günther I, Binder F
Gene. 1982 Sep;19(2):231-4. doi: 10.1016/0378-1119(82)90011-7.
A new plasmid cloning vector (pHE3) is described carrying dominant p-fluorophenylalanine-sensitivity (pheS) and chloramphenicol-resistance markers. This vector is used in combination with a p-fluorophenylalanine-resistant Escherichia coli recipient (strain RR28). Foreign DNA can be cloned into pHE3 leading to insertional inactivation of pheS. Transformation of RR28, and plating on minimal medium with chloramphenicol plus p-fluorophenylalanine (pfp) directly selects for colonies containing recombinant DNA.
描述了一种新的质粒克隆载体（pHE3），其携带显性对氟苯丙氨酸敏感性（pheS）和氯霉素抗性标记。该载体与对氟苯丙氨酸抗性的大肠杆菌受体（RR28菌株）联合使用。外源DNA可克隆到pHE3中，导致pheS插入失活。将RR28进行转化，并接种在含有氯霉素加对氟苯丙氨酸（pfp）的基本培养基上，可直接筛选出含有重组DNA的菌落。
