来自植原体柑橘螺原体的一个质粒在大肠杆菌中的特性鉴定与分子克隆

Mouches C, Barroso G, Bové J M

J Bacteriol. 1983 Nov;156(2):952-5. doi: 10.1128/jb.156.2.952-955.1983.

Two plasmids, pMH1 with 7 kilobase pairs and pM41 with 8 kilobase pairs, were purified from the plant pathogen Spiroplasma citri and characterized by restriction mapping. Upon in vitro DNA recombination with plasmid pBR328 as a vector, we have cloned pMH1 in Escherichia coli. A radioactive probe obtained upon nick translation of the recombinant plasmid was used to further characterize and compare pMH1 and pM41.

从植物病原体柑橘螺原体中纯化出两个质粒，分别是7千碱基对的pMH1和8千碱基对的pM41，并通过限制性酶切图谱分析对其进行了表征。以质粒pBR328作为载体进行体外DNA重组后，我们已将pMH1克隆到大肠杆菌中。通过对重组质粒进行切口平移获得的放射性探针，用于进一步表征和比较pMH1和pM41。