在uncH基因上存在缺陷的大肠杆菌突变体。
Escherichia coli mutants defective in the uncH gene.
作者信息
Humbert R, Brusilow W S, Gunsalus R P, Klionsky D J, Simoni R D
出版信息
J Bacteriol. 1983 Jan;153(1):416-22. doi: 10.1128/jb.153.1.416-422.1983.
Abstract
Plasmids carrying cloned segments of the unc operon of Escherichia coli have been used in genetic complementation analyses to identify three independent mutants defective in the uncH gene, which codes for the delta subunit of the ATP synthetase. Mutations in other unc genes have also been mapped by this technique. ATPase activity was present in extracts of the uncH mutants, but the enzyme was not as tightly bound to the membrane as it was in the parental strain. ATP-dependent membrane energization was absent in membranes isolated from the uncH mutants and could not be restored by adding normal F1 ATPase from the wild-type strain. F1 ATPase prepared from uncH mutants could not restore ATP-dependent membrane energization when added to wild-type membranes depleted of F1. Membranes of the uncH mutants were not rendered proton permeable as a result of washing with low-ionic-strength buffer.
摘要
携带大肠杆菌unc操纵子克隆片段的质粒已用于遗传互补分析，以鉴定uncH基因中有缺陷的三个独立突变体，该基因编码ATP合成酶的δ亚基。其他unc基因中的突变也已通过该技术进行定位。uncH突变体的提取物中存在ATP酶活性，但该酶与膜的结合不如亲本菌株紧密。从uncH突变体中分离的膜中不存在ATP依赖性膜能量化，并且不能通过添加野生型菌株的正常F1 ATP酶来恢复。当添加到耗尽F1的野生型膜中时，从uncH突变体制备的F1 ATP酶不能恢复ATP依赖性膜能量化。用低离子强度缓冲液洗涤后，uncH突变体的膜不会变得质子可渗透。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Linkage map of Escherichia coli K-12, edition 6.大肠杆菌K-12连锁图谱，第6版。
Microbiol Rev. 1980 Mar;44(1):1-56. doi: 10.1128/mr.44.1.1-56.1980.
3
The genes for the eight subunits of the membrane bound ATP synthase of Escherichia coli.大肠杆菌膜结合ATP合酶八个亚基的基因。
Mol Gen Genet. 1981;183(3):463-72. doi: 10.1007/BF00268766.
4
Nucleotide sequence of the gene coding for the delta subunit of proton translocating ATPase of Escherichia coli.大肠杆菌质子转运ATP酶δ亚基编码基因的核苷酸序列。
Biochem Biophys Res Commun. 1981 Sep 16;102(1):172-9. doi: 10.1016/0006-291x(81)91504-7.
5
Assembly of the adenosine triphosphatase complex in Escherichia coli: assembly of F0 is dependent on the formation of specific F1 subunits.大肠杆菌中三磷酸腺苷酶复合物的组装：F0 的组装依赖于特定 F1 亚基的形成。
J Bacteriol. 1981 Oct;148(1):30-42. doi: 10.1128/jb.148.1.30-42.1981.
6
Structural gene coding for the dicyclohexylcarbodiimide-binding protein of the protein-translocating ATPase from Escherichia coli. Locus of the gene in the F1--F0 gene cluster.
FEBS Lett. 1981 May 5;127(1):48-52. doi: 10.1016/0014-5793(81)80338-9.
7
Three genes coding for subunits of the membrane sector (F0) of the Escherichia coli adenosine triphosphatase complex.编码大肠杆菌三磷酸腺苷酶复合体膜部（F0）亚基的三个基因。
J Bacteriol. 1981 Jan;145(1):200-10. doi: 10.1128/jb.145.1.200-210.1981.
8
Subunits of the adenosine triphosphatase complex translated in vitro from the Escherichia coli unc operon.从大肠杆菌unc操纵子体外翻译的三磷酸腺苷酶复合物的亚基。
J Bacteriol. 1980 Jul;143(1):8-17. doi: 10.1128/jb.143.1.8-17.1980.
9
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Proc Natl Acad Sci U S A. 1982 Jan;79(2):320-4. doi: 10.1073/pnas.79.2.320.
10
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Mol Gen Genet. 1981;184(1):33-9. doi: 10.1007/BF00271191.
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