Gay N J, Walker J E
Nucleic Acids Res. 1981 May 11;9(9):2187-94. doi: 10.1093/nar/9.9.2187.
Part of the atp (or unc) operon encoding the alpha, beta, gamma, delta, and epsilon subunits of Escherichia coli ATP-synthase has been cloned into the plasmid pACYC 184. The DNA coding for the largest of these proteins, the alphas subunit, has been sequenced by cloning into the bacteriophage M13 and sequencing with dideoxy nucleotide chain terminators. It comprises 1539 nucleotides corresponding to a protein of 513 amino acids.
编码大肠杆菌ATP合酶α、β、γ、δ和ε亚基的atp(或unc)操纵子的一部分已被克隆到质粒pACYC 184中。通过克隆到噬菌体M13中并用双脱氧核苷酸链终止剂进行测序,对这些蛋白质中最大的α亚基的DNA进行了测序。它由1539个核苷酸组成,对应于一个513个氨基酸的蛋白质。