Njus D, Knoth J, Cook C, Kelly P M
J Biol Chem. 1983 Jan 10;258(1):27-30.
Membrane vesicles (ghosts) containing ascorbic acid were prepared from bovine chromaffin granules. When ferricyanide or ferricytochrome c were added to the external medium, a membrane potential (interior positive) developed across the ghost membrane. This membrane potential could not be elicited from ascorbate-free ghosts or by ferrocyanide added instead of ferricyanide. These results indicate that the chromaffin-granule membrane has a transmembrane electron carrier with a midpoint potential between that of ascorbate (+85 mV) and that of cytochrome c (+255 mV). The most likely candidate is cytochrome b-561 (+140 mV).
含有抗坏血酸的膜泡（血影）由牛嗜铬颗粒制备而成。当向外部介质中添加铁氰化物或铁细胞色素c时，跨血影膜产生了膜电位（内部为正）。这种膜电位无法从不含抗坏血酸的血影中诱发，也不能通过添加亚铁氰化物而非铁氰化物来诱发。这些结果表明，嗜铬颗粒膜具有一种跨膜电子载体，其中点电位介于抗坏血酸（+85 mV）和细胞色素c（+255 mV）的中点电位之间。最有可能的候选者是细胞色素b-561（+140 mV）。
