Bartsch G, Oberholzer M, Holliger O, Weber J, Weber A, Rohr H P
Andrologia. 1978 Jan-Feb;10(1):31-42.
Stereology is a method to obtain quantitative information of structural changes at the light and electron microscopic level. The basic principles of stereology are outlined below. This paper shows how stereologic techniques can be used to obtain structural data from histologic and electron micrographs of intact tissue and cells. A stereologic model, which provides information on the structure of the epididymis has been developed for the rat epididymal head. The model consists of morphologically defined space and membrane compartments of the rat epididymal head and the principal cells. The alterations, induced in the principal cell of the epididymal head after long term hypophysectomy were studied by electron microscopy. The results presented are relative to a cubic centimeter of epididymal tissue, a cubic centimeter of principal cells and principal cell cytoplasm. An attempt to relate stereologic and biochemical data is shown.
体视学是一种在光学和电子显微镜水平上获取结构变化定量信息的方法。下面概述了体视学的基本原理。本文展示了如何运用体视学技术从完整组织和细胞的组织学及电子显微照片中获取结构数据。已为大鼠附睾头部构建了一个提供附睾结构信息的体视学模型。该模型由大鼠附睾头部以及主细胞的形态学定义空间和膜性区室组成。通过电子显微镜研究了长期垂体切除术后附睾头部主细胞所诱导发生的变化。所呈现的结果是相对于一立方厘米附睾组织、一立方厘米主细胞和主细胞细胞质而言的。展示了将体视学数据与生化数据相关联的尝试。
