血管活性肠肽与大鼠和兔肠上皮细胞基底外侧膜的优先结合。
Preferential binding of vasoactive intestinal polypeptide to basolateral membrane of rat and rabbit enterocytes.
作者信息
Dharmsathaphorn K, Harms V, Yamashiro D J, Hughes R J, Binder H J, Wright E M
出版信息
J Clin Invest. 1983 Jan;71(1):27-35. doi: 10.1172/jci110748.
Abstract
Binding of radioiodinated vasoactive intestinal polypeptide (VIP) to intestinal cell membranes of the rabbit ileum and rat jejunum was investigated. Specific binding of 125I-labeled VIP could be demonstrated only on the basolateral membrane and not on the brush border membrane. This corresponded with the lack of an effect on ion transport when VIP was applied to the mucosal side of an in vitro preparation of rabbit ileum. VIP altered ion transport only when it was applied to the serosal side. The binding of 125I-VIP was specific and dependent upon incubation temperature. There was a close correlation between the potency of VIP for inhibition of 125I-VIP binding and that for increasing adenylate cyclase activity. These observations demonstrate that VIP receptors are located on the basolateral membrane.
摘要
研究了放射性碘化血管活性肠肽（VIP）与兔回肠和大鼠空肠肠细胞膜的结合情况。仅在基底外侧膜上可证明125I标记的VIP有特异性结合，而在刷状缘膜上则没有。这与将VIP应用于兔回肠体外制剂的黏膜侧时对离子转运缺乏影响相一致。仅当将VIP应用于浆膜侧时，它才会改变离子转运。125I-VIP的结合是特异性的，且依赖于孵育温度。VIP抑制125I-VIP结合的效力与其增加腺苷酸环化酶活性的效力之间存在密切相关性。这些观察结果表明，VIP受体位于基底外侧膜上。
相似文献
1
Preferential binding of vasoactive intestinal polypeptide to basolateral membrane of rat and rabbit enterocytes.血管活性肠肽与大鼠和兔肠上皮细胞基底外侧膜的优先结合。
J Clin Invest. 1983 Jan;71(1):27-35. doi: 10.1172/jci110748.
2
Presence of vasoactive intestinal peptide receptors coupled to adenylate cyclase in rat lung membranes.
Biochim Biophys Acta. 1981 Nov 18;678(1):76-82. doi: 10.1016/0304-4165(81)90049-0.
3
Binding of vasoactive intestinal peptide and its stimulation of adenylate cyclase through two classes of receptors in rat liver membranes. Effects of 12 secretin analogues and 2 secretin fragments.血管活性肠肽的结合及其通过大鼠肝细胞膜上两类受体对腺苷酸环化酶的刺激作用。12种促胰液素类似物和2种促胰液素片段的作用。
Biochim Biophys Acta. 1981 Nov 18;678(1):83-90. doi: 10.1016/0304-4165(81)90050-7.
4
[Vasoactive intestinal peptide (VIP): specific receptors and adenylate cyclase activation in a human prolactin-secreting pituitary tumor].[血管活性肠肽（VIP）：人催乳素分泌型垂体瘤中的特异性受体与腺苷酸环化酶激活]
C R Seances Acad Sci D. 1979 May 7;288(17):1315-7.
5
Effects of PHI on vasoactive intestinal peptide receptors and adenylate cyclase activity in lung membranes. A comparison in man, rat, mouse and guinea pig.肽组氨酸异亮氨酸对肺膜血管活性肠肽受体及腺苷酸环化酶活性的影响。人、大鼠、小鼠及豚鼠的比较。
Regul Pept. 1982 Oct;4(5):241-50. doi: 10.1016/0167-0115(82)90117-3.
6
Heart receptors for VIP, PHI and secretin are able to activate adenylate cyclase and to mediate inotropic and chronotropic effects. Species variations and physiopathology.血管活性肠肽、胰高血糖素样肽和促胰液素的心脏受体能够激活腺苷酸环化酶并介导变力性和变时性效应。物种差异与病理生理学。
Peptides. 1984 Mar-Apr;5(2):341-53. doi: 10.1016/0196-9781(84)90232-8.
7
Demonstration of a functional receptor for vasoactive intestinal polypeptide on Molt 4b T lymphoblasts.
Regul Pept. 1983 Apr;6(1):1-12. doi: 10.1016/0167-0115(83)90129-5.
8
Receptors for vasoactive intestinal peptide and secretin on small intestinal epithelial cells.小肠上皮细胞上的血管活性肠肽和促胰液素受体。
Am J Physiol. 1980 Mar;238(3):G190-6. doi: 10.1152/ajpgi.1980.238.3.G190.
9
Interaction of vasoactive intestinal peptide with a cell line (HeLa) derived from human carcinoma of the cervix: binding to specific sites and stimulation of adenylate cyclase.血管活性肠肽与人宫颈癌衍生细胞系（HeLa）的相互作用：与特定位点的结合及对腺苷酸环化酶的刺激作用。
Mol Cell Biochem. 1981 Jul;37(3):167-76. doi: 10.1007/BF02354885.
10
Properties of vasoactive intestinal peptide-receptor interaction in rat liver membranes.大鼠肝细胞膜中血管活性肠肽受体相互作用的特性
Rev Esp Fisiol. 1981 Mar;37(1):1-8.
引用本文的文献
1
GLP-2 regulation of intestinal lipid handling.胰高血糖素样肽-2对肠道脂质处理的调节作用。
Front Physiol. 2024 Feb 14;15:1358625. doi: 10.3389/fphys.2024.1358625. eCollection 2024.
2
Colonic delivery of vasoactive intestinal peptide nanomedicine alleviates colitis and shows promise as an oral capsule.肠道递送达肠活性肽纳米药物可缓解结肠炎，并有望成为口服胶囊。
Nanomedicine (Lond). 2020 Oct;15(25):2459-2474. doi: 10.2217/nnm-2020-0280. Epub 2020 Sep 25.
3
Expression and localization of VPAC1, the major receptor of vasoactive intestinal peptide along the length of the intestine.血管活性肠肽主要受体VPAC1在肠道全长中的表达及定位
Am J Physiol Gastrointest Liver Physiol. 2017 Jul 1;313(1):G16-G25. doi: 10.1152/ajpgi.00081.2017. Epub 2017 Apr 6.
4
Inhibition of intestinal dipeptide transport by the neuropeptide VIP is an anti-absorptive effect via the VPAC1 receptor in a human enterocyte-like cell line (Caco-2).神经肽血管活性肠肽（VIP）对肠道二肽转运的抑制作用是通过人肠上皮样细胞系（Caco-2）中的VPAC1受体产生的一种抗吸收效应。
Br J Pharmacol. 2003 Feb;138(4):564-73. doi: 10.1038/sj.bjp.0705049.
5
Oesophageal epithelial innervation in health and reflux oesophagitis.健康与反流性食管炎状态下的食管上皮神经支配
Gut. 1999 Mar;44(3):317-22. doi: 10.1136/gut.44.3.317.
6
Opioid receptors on guinea-pig intestinal crypt epithelial cells.豚鼠肠道隐窝上皮细胞上的阿片受体。
J Physiol. 1996 Nov 15;497 ( Pt 1)(Pt 1):161-74. doi: 10.1113/jphysiol.1996.sp021757.
7
HCl-stimulated duodenal HCO3- secretion in conscious rat. Interactions among VIP, nicotinic receptor mechanisms, and prostaglandins.清醒大鼠中盐酸刺激的十二指肠碳酸氢根分泌。血管活性肠肽、烟碱受体机制和前列腺素之间的相互作用。
Dig Dis Sci. 1994 Oct;39(10):2134-42. doi: 10.1007/BF02090361.
8
Fluid absorption in isolated perfused colonic crypts.离体灌注结肠隐窝中的液体吸收
J Clin Invest. 1995 Nov;96(5):2373-9. doi: 10.1172/JCI118294.
9
Vasoactive intestinal polypeptide regulation of rabbit renal adenylate cyclase activity in vitro.血管活性肠肽对家兔肾腺苷酸环化酶活性的体外调节
J Physiol. 1987 Jun;387:1-17. doi: 10.1113/jphysiol.1987.sp016558.
10
Characterization of specific pancreatic polypeptide receptors on basolateral membranes of the canine small intestine.犬小肠基底外侧膜上特定胰多肽受体的特征分析。
Proc Natl Acad Sci U S A. 1988 Jul;85(13):4745-9. doi: 10.1073/pnas.85.13.4745.
本文引用的文献
1
Preparation of iodine-131 labelled human growth hormone of high specific activity.高比活度碘-131标记人生长激素的制备
Nature. 1962 May 5;194:495-6. doi: 10.1038/194495a0.
2
Pathways for alanine transport in intestinal basal lateral membrane vesicles.肠道基底外侧膜囊泡中丙氨酸转运的途径。
J Membr Biol. 1980 Jan 31;52(1):83-92. doi: 10.1007/BF01869009.
3
Insulin binding and degradation by luminal and basolateral tubular membranes from rabbit kidney.兔肾管腔膜和基底外侧膜对胰岛素的结合与降解
J Clin Invest. 1982 May;69(5):1136-46. doi: 10.1172/jci110549.
4
Effect of vasoactive intestinal polypeptide on active and passive transport in the human jejunum.血管活性肠肽对人空肠主动和被动转运的影响。
J Clin Invest. 1981 Jun;67(6):1687-94. doi: 10.1172/jci110206.
5
In vitro effects of somatostatin on ion transport in rabbit intestine.生长抑素对兔肠道离子转运的体外作用
Am J Physiol. 1980 Feb;238(2):G67-74. doi: 10.1152/ajpgi.1980.238.2.G67.
6
Evidence for and significance of the projection of VIP neurons from the myenteric plexus to the taenia coli in the guinea pig.豚鼠肌间神经丛中血管活性肠肽（VIP）神经元向结肠带投射的证据及意义。
Gastroenterology. 1981 Jun;80(6):1557-61.
7
Interaction of vasoactive intestinal peptide with human blood mononuclear cells.血管活性肠肽与人血单核细胞的相互作用。
Mol Cell Endocrinol. 1981 Feb;21(2):151-60. doi: 10.1016/0303-7207(81)90052-6.
8
Characterization of vasoactive intestinal peptide receptors in human colonic epithelial cells.人结肠上皮细胞中血管活性肠肽受体的特性研究
J Clin Endocrinol Metab. 1981 Apr;52(4):715-21. doi: 10.1210/jcem-52-4-715.
9
Influence of opiates on ion transport across rabbit ileal mucosa.阿片类药物对兔回肠黏膜离子转运的影响。
Gastroenterology. 1981 Feb;80(2):279-84.
10
Sugar uptake by intestinal basolateral membrane vesicles.肠道基底外侧膜囊泡对糖的摄取。
Biochim Biophys Acta. 1980 Mar 27;597(1):112-24. doi: 10.1016/0005-2736(80)90155-8.
Zotero 插件