Sagone A L, Greenwald J, Kraut E H, Bianchine J, Singh D
J Lab Clin Med. 1983 Jan;101(1):97-104.
Recent observations indicate that OH . may be important in the microbicidal capacity of phagocytic cells, in prostaglandin metabolism, and as a mediator of inflammation. Although glucose is a weak hydroxyl scavenger, it occurs in high concentrations in biological systems. We therefore studied the capacity of glucose to scavenge OH . in biological systems known to generate this reactive oxygen species. Our experiments used a specific assay for the detection of OH .. We measured 14CO2 released during the oxidation of 14C-benzoic acid. We have previously demonstrated that benzoic acid is oxidized as a consequence of OH . in the following systems: the enzyme system xanthine-xanthine oxidase, zymosan-stimulated granulocytes, and arachidonic acid-stimulated platelets as a consequence of the lipoxygenase pathway. In all three systems the oxidation of benzoic acid was inversely proportional to the concentration of glucose in the assays. Also, platelets incubated with arachidonic acid and a high concentration of glucose increased HETE production, an effect predicted by the capacity of glucose to act as an OH . scavenger. Our results indicate that glucose acts as a scavenger of OH . in physiological concentrations and therefore may serve an antioxidant role in biological systems. In addition, the capacity of glucose to act as an OH . scavenger may explain some of the defects seen in patients with diabetes mellitus.
最近的观察结果表明，羟基自由基（·OH）可能在吞噬细胞的杀菌能力、前列腺素代谢以及作为炎症介质方面发挥重要作用。尽管葡萄糖是一种较弱的羟基清除剂，但它在生物系统中以高浓度存在。因此，我们研究了葡萄糖在已知会产生这种活性氧的生物系统中清除·OH的能力。我们的实验使用了一种检测·OH的特定方法。我们测量了14C-苯甲酸氧化过程中释放的14CO2。我们之前已经证明，在以下系统中，苯甲酸会因·OH而被氧化：黄嘌呤-黄嘌呤氧化酶酶系统、酵母聚糖刺激的粒细胞以及脂氧合酶途径导致的花生四烯酸刺激的血小板。在所有这三个系统中，苯甲酸的氧化与测定中葡萄糖的浓度成反比。此外，用花生四烯酸和高浓度葡萄糖孵育的血小板增加了羟二十碳四烯酸（HETE）的产生，这一效应可由葡萄糖作为·OH清除剂的能力来预测。我们的结果表明，葡萄糖在生理浓度下起·OH清除剂的作用，因此可能在生物系统中起到抗氧化作用。此外，葡萄糖作为·OH清除剂的能力可能解释了糖尿病患者中出现的一些缺陷。
