Rassoulzadegan M, Cowie A, Carr A, Glaichenhaus N, Kamen R, Cuzin F
Nature. 1982 Dec 23;300(5894):713-8. doi: 10.1038/300713a0.
The expression in normal rat cells of modified polyoma virus genomes, separately encoding large T, middle T or small T antigens, has allowed the investigation of the roles of these proteins in oncogenic transformation. Middle T is sufficient to transform cells of established lines but the transformants are serum dependent. Large T lacks intrinsic oncogenic potential but can relieve the serum dependence of normal and transformed cells. Middle T alone cannot transform primary rat embryo fibroblasts.
分别编码大T、中T或小T抗原的修饰多瘤病毒基因组在正常大鼠细胞中的表达，使得对这些蛋白质在致癌转化中的作用进行研究成为可能。中T足以转化已建立细胞系的细胞，但转化细胞依赖血清。大T缺乏内在的致癌潜力，但可以解除正常细胞和转化细胞对血清的依赖性。单独的中T不能转化原代大鼠胚胎成纤维细胞。
