Chowdhury K, Meltzer M L, Israel M A
J Virol. 1982 Mar;41(3):1000-6. doi: 10.1128/JVI.41.3.1000-1006.1982.
To determine the stability of polyoma viral DNA in transformed rat cells during their growth in vivo, we compared the state and arrangement of polyoma virus DNA sequences in virus-transformed rat cell lines before and after their passage in vivo. In cell lines from 12 independent tumors induced by the inoculation of animals with three different transformed cell lines, we could detect no significant changes in the arrangement of viral DNA sequences associated with the in vivo passage of these cell lines. In 13 of 14 tumor cell lines examined, the pattern of polyoma virus tumor antigens, characterized by the presence of the polyoma virus large, middle, and small tumor antigens, was unchanged.
为了确定多瘤病毒DNA在转化的大鼠细胞体内生长过程中的稳定性，我们比较了多瘤病毒转化的大鼠细胞系在体内传代前后病毒DNA序列的状态和排列。在用三种不同的转化细胞系接种动物诱导产生的12个独立肿瘤的细胞系中，我们未检测到与这些细胞系体内传代相关的病毒DNA序列排列有显著变化。在检测的14个肿瘤细胞系中的13个中，以多瘤病毒大、中、小肿瘤抗原的存在为特征的多瘤病毒肿瘤抗原模式未发生变化。
