果蝇拓扑异构酶I：分离、纯化与特性分析。
Drosophila topoisomerase I: isolation, purification and characterization.
作者信息
Javaherian K, Tse Y C, Vega J
出版信息
Nucleic Acids Res. 1982 Nov 11;10(21):6945-55. doi: 10.1093/nar/10.21.6945.
Abstract
We have purified and characterized topoisomerase I from Drosophila melanogaster. The molecular weight of the enzyme is 135,000; 100,000, 90,000, and 65,000 molecular weight products result from degradation of the enzyme. The enzyme relaxes both positive and negative supercoiled DNA. Mg++ is not absolutely required, but stimulates the enzymatic activity considerably.
摘要
我们已经从黑腹果蝇中纯化并鉴定了拓扑异构酶I。该酶的分子量为135,000；分子量为100,000、90,000和65,000的产物是该酶降解产生的。该酶可使正超螺旋和负超螺旋DNA松弛。Mg++并非绝对必需，但能显著刺激酶活性。
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