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果蝇DNA拓扑异构酶I与基因组的转录活性区域相关联。

Drosophila DNA topoisomerase I is associated with transcriptionally active regions of the genome.

作者信息

Fleischmann G, Pflugfelder G, Steiner E K, Javaherian K, Howard G C, Wang J C, Elgin S C

出版信息

Proc Natl Acad Sci U S A. 1984 Nov;81(22):6958-62. doi: 10.1073/pnas.81.22.6958.

DOI:10.1073/pnas.81.22.6958
PMID:6095263
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC392055/
Abstract

The distribution of DNA topoisomerase I within Drosophila polytene chromosomes was observed by immunofluorescent staining with affinity-purified antibodies. The enzyme is preferentially associated with active loci, as shown by prominent staining of puffs. The heat shock loci 87A-87C are stained after, but not before, heat shock induction. A detailed comparison of the distribution of topoisomerase I with that of RNA polymerase II reveals a similar, although not identical, pattern of association. Topoisomerase I is also found in association with the nucleolus, the site of transcription by RNA polymerase I.

摘要

用亲和纯化抗体进行免疫荧光染色，观察了果蝇多线染色体中DNA拓扑异构酶I的分布。如疏松区显著染色所示，该酶优先与活性位点相关联。热休克基因座87A - 87C在热休克诱导后而非诱导前被染色。对拓扑异构酶I与RNA聚合酶II分布的详细比较揭示了一种相似但不完全相同的关联模式。拓扑异构酶I也被发现与核仁相关联，核仁是RNA聚合酶I进行转录的位点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91c3/392055/d53c61402d27/pnas00623-0060-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91c3/392055/6bbe5c7784f6/pnas00623-0058-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91c3/392055/886b0dde5cc8/pnas00623-0058-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91c3/392055/a5a15aa8317a/pnas00623-0059-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91c3/392055/72d4604d08d8/pnas00623-0059-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91c3/392055/01afd42b9cf0/pnas00623-0059-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91c3/392055/d53c61402d27/pnas00623-0060-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91c3/392055/6bbe5c7784f6/pnas00623-0058-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91c3/392055/886b0dde5cc8/pnas00623-0058-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91c3/392055/a5a15aa8317a/pnas00623-0059-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91c3/392055/72d4604d08d8/pnas00623-0059-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91c3/392055/01afd42b9cf0/pnas00623-0059-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91c3/392055/d53c61402d27/pnas00623-0060-a.jpg

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本文引用的文献

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[The puffs of salivary gland chromosomes of Drosophilia melanogaster. Part 1. Observations on the behavior of a typical puff in the normal strain and in two mutants, giant and lethal giant larvae].[黑腹果蝇唾液腺染色体的胀泡。第1部分。关于正常品系以及两种突变体（巨型和致死巨型幼虫）中典型胀泡行为的观察]
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Diazotizable arylamine cellulose papers for the coupling and hybridization of nucleic acids.用于核酸偶联和杂交的重氮化芳胺纤维素纸。
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Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples.
Mol Cell. 2016 May 5;62(3):327-334. doi: 10.1016/j.molcel.2016.03.034.
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Recruitment timing and dynamics of transcription factors at the Hsp70 loci in living cells.活细胞中 HSP70 基因座转录因子的募集时间和动态。
Mol Cell. 2010 Dec 22;40(6):965-75. doi: 10.1016/j.molcel.2010.11.022.
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The SR protein B52/SRp55 is required for DNA topoisomerase I recruitment to chromatin, mRNA release and transcription shutdown.SR 蛋白 B52/SRp55 对于 DNA 拓扑异构酶 I 向染色质的募集、mRNA 释放和转录终止是必需的。
PLoS Genet. 2010 Sep 16;6(9):e1001124. doi: 10.1371/journal.pgen.1001124.
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Topoisomerase I regulates open chromatin and controls gene expression in vivo.拓扑异构酶 I 调节开放染色质并在体内控制基因表达。
EMBO J. 2010 Jul 7;29(13):2126-34. doi: 10.1038/emboj.2010.109. Epub 2010 Jun 4.
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Mutational analysis of the preferential binding of human topoisomerase I to supercoiled DNA.人拓扑异构酶I与超螺旋DNA优先结合的突变分析
FEBS J. 2009 Oct;276(20):5906-19. doi: 10.1111/j.1742-4658.2009.07270.x. Epub 2009 Sep 9.
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10
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