Fleischmann G, Pflugfelder G, Steiner E K, Javaherian K, Howard G C, Wang J C, Elgin S C
Proc Natl Acad Sci U S A. 1984 Nov;81(22):6958-62. doi: 10.1073/pnas.81.22.6958.
The distribution of DNA topoisomerase I within Drosophila polytene chromosomes was observed by immunofluorescent staining with affinity-purified antibodies. The enzyme is preferentially associated with active loci, as shown by prominent staining of puffs. The heat shock loci 87A-87C are stained after, but not before, heat shock induction. A detailed comparison of the distribution of topoisomerase I with that of RNA polymerase II reveals a similar, although not identical, pattern of association. Topoisomerase I is also found in association with the nucleolus, the site of transcription by RNA polymerase I.
用亲和纯化抗体进行免疫荧光染色，观察了果蝇多线染色体中DNA拓扑异构酶I的分布。如疏松区显著染色所示，该酶优先与活性位点相关联。热休克基因座87A - 87C在热休克诱导后而非诱导前被染色。对拓扑异构酶I与RNA聚合酶II分布的详细比较揭示了一种相似但不完全相同的关联模式。拓扑异构酶I也被发现与核仁相关联，核仁是RNA聚合酶I进行转录的位点。
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