Lazarus L H, DiAugustine R P, Jahnke G D, Hernandez O
Science. 1983 Jan 7;219(4580):79-81. doi: 10.1126/science.6294828.
Immunoreactivity to the amphibian peptide physalaemin was characterized from extracts of a human lung small-cell carcinoma by immunological, chemical, and pharmacological means. Tumor-related peptide cross-reacted with three antiserums to physalaemin to yield 1.1 to 1.6 nanomoles per gram of tissue. Physalaemin and tumor peptide had similar retention times on high-performance liquid chromatography after chemical and enzymic modifications that included pH changes, oxone oxidation, use of a hydrophilic ion-pairing reagent, and digestion with trypsin and pyroglutamate aminopeptidase. Both physalaemin and the tumor peptide produced a contractile response of isolated guinea pig ileum at threshold concentrations of approximately 100 to 150 picograms per milliliter. These data suggest that small-cell carcinoma of the lung contains a physalaemin-like peptide that has structural and biological homology to its amphibian counterpart.
通过免疫学、化学和药理学方法，对人肺小细胞癌提取物中对两栖类肽 Physalaemin 的免疫反应性进行了表征。肿瘤相关肽与三种针对 Physalaemin 的抗血清发生交叉反应，每克组织产生 1.1 至 1.6 纳摩尔的反应。在进行包括 pH 变化、过氧单硫酸盐氧化、使用亲水性离子对试剂以及用胰蛋白酶和焦谷氨酸氨肽酶消化等化学和酶修饰后，Physalaemin 和肿瘤肽在高效液相色谱上具有相似的保留时间。Physalaemin 和肿瘤肽在约 100 至 150 皮克/毫升的阈值浓度下均能使分离的豚鼠回肠产生收缩反应。这些数据表明，肺小细胞癌含有一种与两栖类 Physalaemin 具有结构和生物学同源性的类似肽。
