Singleton F L, Attwell R, Jangi S, Colwell R R
Appl Environ Microbiol. 1982 Nov;44(5):1047-58. doi: 10.1128/aem.44.5.1047-1058.1982.
Laboratory microecosystems (microcosms) prepared with a chemically defined sea salt solution were used to study effects of selected environmental parameters on growth and activity of Vibrio cholerae. Growth responses under simulated estuarine conditions of 10 strains of V. cholerae, including clinical and environmental isolates as well as serovars O1 and non-O1, were compared, and all strains yielded populations of approximately the same final size. Effects of salinity and temperature on extended survival of V. cholerae demonstrated that, at an estuarine salinity (25%) and a temperature of 10 degrees C, V. cholerae survived (i.e., was culturable) for less than 4 days. Salinity was also found to influence activity, as measured by uptake of 14C-amino acids. Studies on the effect of selected ions on growth and activity of V. cholerae demonstrated that Na+ was required for growth. The results of this study further support the status of V. cholerae as an estuarine bacterium.
用化学定义的海盐溶液制备的实验室微生态系统（微观世界）用于研究选定环境参数对霍乱弧菌生长和活性的影响。比较了10株霍乱弧菌在模拟河口条件下的生长反应，这些菌株包括临床分离株和环境分离株以及O1和非O1血清型，所有菌株最终产生的菌量大致相同。盐度和温度对霍乱弧菌长期存活的影响表明，在河口盐度（25%）和10摄氏度的温度下，霍乱弧菌存活（即可培养）不到4天。还发现盐度会影响活性，这通过14C-氨基酸的摄取来衡量。关于选定离子对霍乱弧菌生长和活性影响的研究表明，生长需要Na+。本研究结果进一步支持了霍乱弧菌作为河口细菌的地位。
