Jackson B J, Kennedy E P
J Biol Chem. 1983 Feb 25;258(4):2394-8.
Membrane-derived oligosaccharides, found in the Escherichia coli periplasmic space (Schulman, H., and Kennedy, E. P. (1979) J. Bacteriol. 137, 686-688), are composed of 8-10 units of glucose, the sole sugar, in beta 1 leads to 2 and beta 1 leads to 6 linkages (Schneider, J. E., Reinhold, V., Rumley, M. K., and Kennedy, E. P. (1979) J. Biol. Chem. 254, 10135-10138). Oligosaccharides in this family are variously substituted with succinyl ester residues, as well as with sn-1-phosphoglycerol and phosphoethanolamine, both derived from membrane phospholipids. These negatively charged oligosaccharides may function in cellular osmoregulation since their synthesis is under osmotic control (Kennedy, E. P. (1982) Proc. Natl. Acad. Sci. U.S.A. 79, 1092-1095). We now report initial characterization of an enzyme catalyzing transfer of phosphoglycerol residues from phosphatidylglycerol to membrane-derived oligosaccharides or to synthetic beta-glucoside acceptors. The products are sn-1,2-diglyceride and beta-glucoside-6-phosphoglycerol. Localized in the inner membrane, the transferase has a requirement for divalent cations, of which manganese is most effective, and a pH optimum of 8.9 in vitro.
膜衍生寡糖存在于大肠杆菌周质空间（舒尔曼，H.，和肯尼迪，E.P.（1979年）《细菌学杂志》137卷，686 - 688页），由8 - 10个葡萄糖单元组成，葡萄糖是唯一的糖类，以β1→2和β1→6连接（施耐德，J.E.，莱因霍尔德，V.，拉姆利，M.K.，和肯尼迪，E.P.（1979年）《生物化学杂志》254卷，10135 - 10138页）。这个家族的寡糖被琥珀酰酯残基以及均来源于膜磷脂的sn - 1 - 磷酸甘油和磷酸乙醇胺以各种方式取代。这些带负电荷的寡糖可能在细胞渗透调节中发挥作用，因为它们的合成受渗透压控制（肯尼迪，E.P.（1982年）《美国国家科学院院刊》79卷，1092 - 1095页）。我们现在报告一种酶的初步特性，该酶催化磷酸甘油残基从磷脂酰甘油转移到膜衍生寡糖或合成的β - 葡萄糖苷受体上。产物是sn - 1,2 - 甘油二酯和β - 葡萄糖苷 - 6 - 磷酸甘油。该转移酶定位于内膜，需要二价阳离子，其中锰最为有效，体外最适pH为8.9。
