Stohlman S A, Brayton P R, Fleming J O, Weiner L P, Lai M M
J Gen Virol. 1982 Dec;63(2):265-75. doi: 10.1099/0022-1317-63-2-265.
Two plaque-size variants of the neurotropic JHM strain of mouse hepatitis virus have been isolated from the virus stock after eight serial passages in suckling mouse brain. One variant, JHM-DL, produces large plaques, while the other, JHM-DS, produces small plaques in tissue culture. DS replicates more slowly, has a lower virus yield in vitro, and is less virulent for mice than DL. They also differ in their pathogenicity for mice: JHM-DL infection results in acute encephalomyelitis while JHM-DS infection results in demyelination. Oligonucleotide fingerprint analysis of the RNA genomes of these two variants revealed that they had almost identical genetic sequences. Each variant, however, had a unique oligonucleotide spot not found in the other. The unique spot of the large plaque variant, JHM-DL, was localized at approximately 3 to 5 kb from the 3' end, while the JHM-DS unique spot was mapped at 14 to 15 kb from the 3' end of the genome. We have further shown that these oligonucleotide changes are not correlated with the plaque morphology. These two viruses may be useful for studying the molecular basis of virus-induced demyelination.
从小鼠肝炎病毒嗜神经JHM株的病毒原液中，经乳鼠脑内连续传代8次后分离出两种噬斑大小不同的变异株。一种变异株JHM-DL产生大噬斑，另一种变异株JHM-DS在组织培养中产生小噬斑。DS复制较慢，体外病毒产量较低，对小鼠的毒力也比DL弱。它们对小鼠的致病性也有所不同：JHM-DL感染导致急性脑脊髓炎，而JHM-DS感染导致脱髓鞘。对这两种变异株RNA基因组的寡核苷酸指纹分析表明，它们的基因序列几乎相同。然而，每个变异株都有一个在另一个变异株中未发现的独特寡核苷酸斑点。大噬斑变异株JHM-DL的独特斑点位于距3'端约3至5 kb处，而JHM-DS的独特斑点位于基因组3'端14至15 kb处。我们进一步表明，这些寡核苷酸变化与噬斑形态无关。这两种病毒可能有助于研究病毒诱导脱髓鞘的分子基础。
