Roseto A, Scherrer R, Cohen J, Guillemin M C, Charpilienne A, Feynerol C, Peries J
J Gen Virol. 1983 Jan;64 (Pt 1):237-40. doi: 10.1099/0022-1317-64-1-237.
Five monoclonal hybridoma cell lines secreting antibodies against bovine rotavirus have been produced and four of them characterized by immunostaining of structural polypeptides electrophoretically transferred on to nitrocellulose sheets. Three hybridomas appeared to be directed against the major structural polypeptide (VP39) of the virion. These three monoclonals cross-reacted with the major polypeptide of simian rotavirus and human rotavirus. A fourth hybridoma appeared to react specifically with the high-molecular weight external polypeptide (VP89) and its cleavage products. A cross-reaction was observed with human Wa strain but not with SA11. The fifth hybridoma, even though reacting in an immunofluorescent test, did not show any reactivity by immunostaining. None of the monoclonals neutralized the infectivity of bovine rotavirus.
已产生了五种分泌抗牛轮状病毒抗体的单克隆杂交瘤细胞系，其中四种通过对电泳转移至硝酸纤维素膜上的结构多肽进行免疫染色来表征。三种杂交瘤似乎针对病毒粒子的主要结构多肽（VP39）。这三种单克隆抗体与猿猴轮状病毒和人轮状病毒的主要多肽发生交叉反应。第四种杂交瘤似乎与高分子量外部多肽（VP89）及其裂解产物特异性反应。观察到与人Wa株有交叉反应，但与SA11没有交叉反应。第五种杂交瘤尽管在免疫荧光试验中有反应，但通过免疫染色未显示任何反应性。没有一种单克隆抗体能中和牛轮状病毒的感染性。
