六种检测粪便中人类轮状病毒方法的比较。
Comparison of six methods for detecting human rotavirus in stools.
作者信息
Morinet F, Ferchal F, Colimon R, Pérol Y
出版信息
Eur J Clin Microbiol. 1984 Apr;3(2):136-40. doi: 10.1007/BF02014331.
Abstract
The following six methods for detecting rotavirus in human faecal samples were compared: electron microscopy, immune electron microscopy, immunofluorescence in cell culture, two enzyme immunoassays (Rotazyme, Enzygnost ) and a latex agglutination test ( Rotalex ). Specimens were collected from 112 children with diarrhoea. The relative sensitivities of the different assays for human rotavirus were as follows: electron microscopy, 84%; immunofluorescence, 86%; Rotalex , 88%; Rotazyme, 89%; immune electron microscopy, 93%; Enzygnost , 98%. According to our findings Enzygnost is the most sensitive method, but Rotalex is more valuable for screening a small number of faecal samples. No false-positive results were observed in the two enzyme immunoassays or in Rotalex .
摘要
对检测人类粪便样本中轮状病毒的以下六种方法进行了比较
电子显微镜检查、免疫电子显微镜检查、细胞培养中的免疫荧光检查、两种酶免疫测定法（Rotazyme、Enzygnost）和乳胶凝集试验（Rotalex）。从112名腹泻儿童中采集了样本。不同检测方法对人轮状病毒的相对敏感性如下：电子显微镜检查为84%；免疫荧光检查为86%；Rotalex为88%；Rotazyme为89%；免疫电子显微镜检查为93%；Enzygnost为98%。根据我们的研究结果，Enzygnost是最敏感的方法，但Rotalex对少量粪便样本的筛查更有价值。在两种酶免疫测定法或Rotalex中均未观察到假阳性结果。
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