Torfason E G, Diderholm H
J Med Virol. 1982;10(3):157-70. doi: 10.1002/jmv.1890100302.
A method for the absorption of false radioimmunoassay (RIA) IgM titres against herpes simplex virus (HSV) and cytomegalovirus (CMV) is presented. The serum specimens were absorbed by a mixture of protein A-Sepharose and protein A-Sepharose saturated with normal human gamma globulin (PAS/IgG). The detection of rheumatoid factor of IgM class (IgM-RF) as well as antinuclear antibodies (ANA) of both IgM and IgG class by solid-phase RIA is also described, and their role in the false IgM results was studied. It was found that the PAS/IgG absorption removed 50-90% of both IgM-RF and total IgG. The reduction of IgM-ANA clustered at 50-90% or nothing, whereas the reduction of IgG-ANA was approximately 50%. The studies with HSV and CMV antigens indicated that the removal of false IgM titres was more effective than the removal of each of these four factors. It was concluded that the IgM-RF titres alone were not sufficiently high to explain the false IgM results, but the ANA activity probably contributed.
本文介绍了一种针对单纯疱疹病毒（HSV）和巨细胞病毒（CMV）的假放射免疫分析（RIA）IgM滴度的吸收方法。血清标本用蛋白A-琼脂糖和饱和正常人γ球蛋白的蛋白A-琼脂糖混合物（PAS/IgG）进行吸收。还描述了通过固相RIA检测IgM类类风湿因子（IgM-RF）以及IgM和IgG类抗核抗体（ANA），并研究了它们在假IgM结果中的作用。发现PAS/IgG吸收去除了50%-90%的IgM-RF和总IgG。IgM-ANA的减少集中在50%-90%或无减少，而IgG-ANA的减少约为50%。对HSV和CMV抗原的研究表明，去除假IgM滴度比去除这四个因素中的任何一个更有效。得出的结论是，单独的IgM-RF滴度不足以解释假IgM结果，但ANA活性可能起了作用。
