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使用经蛋白A处理的血清来揭示单纯疱疹病毒1型（HSV-1）免疫球蛋白A，并将HSV-1免疫球蛋白G鉴定为主要的中和抗体。

Use of protein A-treated sera in unmasking herpes simplex virus type 1 (HSV-1) immunoglobulin A and identifying HSV-1 immunoglobulin G as the predominant neutralizing antibody.

作者信息

Ratner J J, Sanford B A, Smith K O

出版信息

J Clin Microbiol. 1979 Oct;10(4):415-8. doi: 10.1128/jcm.10.4.415-418.1979.

DOI:10.1128/jcm.10.4.415-418.1979
PMID:231047
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC273189/
Abstract

Treatment of human sera with protein A reduced the amounts of immunoglobulin G (IgG), IgA, and IgM detected by radial immunodiffusion. This treatment also decreased the amount of herpes-specific IgG and IgM detected by radioimmunoassay, whereas it increased and even unmasked the amount of herpes-specific IgA detected. Comparison of protein A-treated sera with untreated sera indicated that herpes simplex virus type 1 IgG was responsible for more than 92 to 99% of the serum neutralizing activity.

摘要

用蛋白A处理人血清会减少通过放射免疫扩散法检测到的免疫球蛋白G（IgG）、IgA和IgM的量。这种处理还会降低通过放射免疫测定法检测到的疱疹特异性IgG和IgM的量，而它会增加甚至揭示出所检测到的疱疹特异性IgA的量。将用蛋白A处理的血清与未处理的血清进行比较表明，1型单纯疱疹病毒IgG占血清中和活性的92%至99%以上。

相似文献

1
Use of protein A-treated sera in unmasking herpes simplex virus type 1 (HSV-1) immunoglobulin A and identifying HSV-1 immunoglobulin G as the predominant neutralizing antibody.使用经蛋白A处理的血清来揭示单纯疱疹病毒1型（HSV-1）免疫球蛋白A，并将HSV-1免疫球蛋白G鉴定为主要的中和抗体。
J Clin Microbiol. 1979 Oct;10(4):415-8. doi: 10.1128/jcm.10.4.415-418.1979.
2
Immunoglobulin class of antibody to herpes simplex virus in patients with oral cancer.口腔癌患者中针对单纯疱疹病毒的抗体免疫球蛋白类别。
Cancer. 1983 Jan 1;51(1):65-71. doi: 10.1002/1097-0142(19830101)51:1<65::aid-cncr2820510116>3.0.co;2-u.
3
IgG-, IgA- and IgM-antibodies to herpes simplex virus type 2 in sera from patients with cancer of the uterine cervix.子宫颈癌患者血清中针对2型单纯疱疹病毒的IgG、IgA和IgM抗体
Eur J Cancer (1965). 1977 Mar 29;13(3):247-51. doi: 10.1016/0014-2964(77)90212-2.
4
Specific IgG and IgA antibodies to herpes simplex virus (HSV)-induced surface antigen in patients with HSV infections and in healthy adults.单纯疱疹病毒（HSV）感染患者及健康成年人中针对HSV诱导的表面抗原的特异性IgG和IgA抗体。
J Med Virol. 1984;14(3):201-7. doi: 10.1002/jmv.1890140303.
5
ELISA for detection of IgG and IgM antibodies to HSV-1 and HSV-2 in human sera.用于检测人血清中抗单纯疱疹病毒1型和2型IgG及IgM抗体的酶联免疫吸附测定
J Virol Methods. 1986 Aug;14(1):43-55. doi: 10.1016/0166-0934(86)90006-6.
6
Use of enzyme-labeled antigen for the detection of immunoglobulin M and A antibody to herpes simplex virus in serum and cerebrospinal fluid.酶标记抗原用于检测血清和脑脊液中单纯疱疹病毒免疫球蛋白M和A抗体。
J Med Virol. 1985 Feb;15(2):183-95. doi: 10.1002/jmv.1890150211.
7
Enzyme-linked immunosorbent assay (ELISA) for detection of herpes simplex virus-specific IgM antibodies.
J Virol Methods. 1982 May;4(4-5):219-27. doi: 10.1016/0166-0934(82)90068-4.
8
False RIA IgM titres to herpes simplex virus and cytomegalovirus: factors causing them, and their absorption by protein A-Sepharose/IgG-protein A-Sepharose.单纯疱疹病毒和巨细胞病毒的假放射免疫分析IgM滴度：产生假滴度的因素以及它们被蛋白A-琼脂糖/IgG-蛋白A-琼脂糖吸附的情况
J Med Virol. 1982;10(3):157-70. doi: 10.1002/jmv.1890100302.
9
Herpes simplex virus-specific serum immunoglobulin a: detection in patients with primary or recurrent herpes infections and in healthy adults.单纯疱疹病毒特异性血清免疫球蛋白A：在原发性或复发性疱疹感染患者及健康成年人中的检测
Infect Immun. 1982 Jul;37(1):374-7. doi: 10.1128/iai.37.1.374-377.1982.
10
Solid-phase radioimmunoassay of human immunoglobulin M and immunoglobulin G antibodies against herpes simplex virus type 1 capsid, envelope, and excreted antigens.针对1型单纯疱疹病毒衣壳、包膜及分泌抗原的人免疫球蛋白M和免疫球蛋白G抗体的固相放射免疫测定
Infect Immun. 1977 Mar;15(3):883-9. doi: 10.1128/iai.15.3.883-889.1977.

引用本文的文献

1
Secretory IgA specific for herpes simplex virus in lacrimal fluid from patients with herpes keratitis--a possible diagnostic parameter.疱疹性角膜炎患者泪液中针对单纯疱疹病毒的分泌型 IgA——一种可能的诊断参数。
Br J Ophthalmol. 1982 Oct;66(10):648-53. doi: 10.1136/bjo.66.10.648.
2
Herpes simplex virus-specific serum immunoglobulin a: detection in patients with primary or recurrent herpes infections and in healthy adults.单纯疱疹病毒特异性血清免疫球蛋白A：在原发性或复发性疱疹感染患者及健康成年人中的检测
Infect Immun. 1982 Jul;37(1):374-7. doi: 10.1128/iai.37.1.374-377.1982.
3
Virus-specific immunoglobulin G subclasses in herpes simplex and varicella-zoster virus infections.单纯疱疹病毒和水痘-带状疱疹病毒感染中的病毒特异性免疫球蛋白G亚类
J Clin Microbiol. 1984 Jul;20(1):94-8. doi: 10.1128/jcm.20.1.94-98.1984.
4
Neutralization capacity and antibody dependent cell-mediated cytotoxicity of separated IgG subclasses 1, 3 and 4 against herpes simplex virus.分离出的IgG亚类1、3和4针对单纯疱疹病毒的中和能力及抗体依赖性细胞介导的细胞毒性
Clin Exp Immunol. 1988 May;72(2):211-5.
5
Establishment by enzyme-linked immunosorbent assay of seronegative range for herpes simplex virus and cytomegalovirus antibodies and evaluation of heterologous responses to live varicella vaccine.通过酶联免疫吸附测定法确定单纯疱疹病毒和巨细胞病毒抗体的血清阴性范围，并评估对水痘减毒活疫苗的异源反应。
J Clin Microbiol. 1988 Apr;26(4):781-3. doi: 10.1128/jcm.26.4.781-783.1988.

本文引用的文献

1
Primary herpes simplex virus infection of the adult, with a note on the relation of herpes simplex virus to recurrent aphthous stomatitis.成人原发性单纯疱疹病毒感染，并论及单纯疱疹病毒与复发性阿弗他口炎的关系
AMA Arch Intern Med. 1951 Oct;88(4):495-502. doi: 10.1001/archinte.1951.03810100079007.
2
Recurrent "fever blister" and "canker sore". Tests for herpes simplex and other viruses with mammalian cell cultures.复发性“唇疱疹”和“口腔溃疡”。采用哺乳动物细胞培养法对单纯疱疹病毒及其他病毒进行检测。
Arch Oral Biol. 1961 Feb;3:117-24. doi: 10.1016/0003-9969(61)90144-3.
3
Diagnosis of recent Herpes simplex infections. A modified immunofluorescent test for the detection of specific Herpes simplex IgM antibodies after staphylococcal adsorption of IgG.近期单纯疱疹感染的诊断。一种经改良的免疫荧光试验，用于在葡萄球菌吸附IgG后检测特异性单纯疱疹IgM抗体。
Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Jun;82(3):323-8.
4
A prospective study of chronic herpes simplex virus infection and recurrent herpes labialis in humans.一项关于人类慢性单纯疱疹病毒感染和复发性唇疱疹的前瞻性研究。
J Immunol. 1970 Feb;104(2):289-95.
5
Radioimmunoassay techniques for detecting naturally occurring viral antibody in human sera.用于检测人血清中天然存在的病毒抗体的放射免疫分析技术。
J Immunol Methods. 1974 Oct;5(4):337-44. doi: 10.1016/0022-1759(74)90017-9.
6
Specific IgG and IgM antibody responses in herpes-simplex-virus infections.
J Med Microbiol. 1974 Aug;7(3):333-41. doi: 10.1099/00222615-7-3-333.
7
Further investigation of specific and non-specific adsorption of serum globulins to Staphylococcus aureus.血清球蛋白对金黄色葡萄球菌的特异性和非特异性吸附的进一步研究。
Acta Pathol Microbiol Scand. 1968;73(4):637-45. doi: 10.1111/j.1699-0463.1968.tb03221.x.
8
A possible role of gamma-A-immunoglobulin in herpes simplex virus infection in man.γ-A-免疫球蛋白在人类单纯疱疹病毒感染中的可能作用。
J Immunol. 1966 Aug;97(2):248-59.
9
A method of trace iodination of proteins for immunologic studies.一种用于免疫学研究的蛋白质微量碘化方法。
Int Arch Allergy Appl Immunol. 1966;29(2):185-9. doi: 10.1159/000229699.
10
Magnetic transfer devices for use in solid-phase radioimmunoassays and enzyme-linked immunosorbent assays.用于固相放射免疫测定和酶联免疫吸附测定的磁性转移装置。
J Infect Dis. 1977 Oct;136 Suppl:S329-36. doi: 10.1093/infdis/136.supplement_2.s329.