使用经蛋白A处理的血清来揭示单纯疱疹病毒1型（HSV-1）免疫球蛋白A，并将HSV-1免疫球蛋白G鉴定为主要的中和抗体。
Use of protein A-treated sera in unmasking herpes simplex virus type 1 (HSV-1) immunoglobulin A and identifying HSV-1 immunoglobulin G as the predominant neutralizing antibody.
作者信息
Ratner J J, Sanford B A, Smith K O
出版信息
J Clin Microbiol. 1979 Oct;10(4):415-8. doi: 10.1128/jcm.10.4.415-418.1979.
Abstract
Treatment of human sera with protein A reduced the amounts of immunoglobulin G (IgG), IgA, and IgM detected by radial immunodiffusion. This treatment also decreased the amount of herpes-specific IgG and IgM detected by radioimmunoassay, whereas it increased and even unmasked the amount of herpes-specific IgA detected. Comparison of protein A-treated sera with untreated sera indicated that herpes simplex virus type 1 IgG was responsible for more than 92 to 99% of the serum neutralizing activity.
摘要
用蛋白A处理人血清会减少通过放射免疫扩散法检测到的免疫球蛋白G（IgG）、IgA和IgM的量。这种处理还会降低通过放射免疫测定法检测到的疱疹特异性IgG和IgM的量，而它会增加甚至揭示出所检测到的疱疹特异性IgA的量。将用蛋白A处理的血清与未处理的血清进行比较表明，1型单纯疱疹病毒IgG占血清中和活性的92%至99%以上。
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Use of protein A-treated sera in unmasking herpes simplex virus type 1 (HSV-1) immunoglobulin A and identifying HSV-1 immunoglobulin G as the predominant neutralizing antibody.使用经蛋白A处理的血清来揭示单纯疱疹病毒1型（HSV-1）免疫球蛋白A，并将HSV-1免疫球蛋白G鉴定为主要的中和抗体。
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本文引用的文献
1
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AMA Arch Intern Med. 1951 Oct;88(4):495-502. doi: 10.1001/archinte.1951.03810100079007.
2
Recurrent "fever blister" and "canker sore". Tests for herpes simplex and other viruses with mammalian cell cultures.复发性“唇疱疹”和“口腔溃疡”。采用哺乳动物细胞培养法对单纯疱疹病毒及其他病毒进行检测。
Arch Oral Biol. 1961 Feb;3:117-24. doi: 10.1016/0003-9969(61)90144-3.
3
Diagnosis of recent Herpes simplex infections. A modified immunofluorescent test for the detection of specific Herpes simplex IgM antibodies after staphylococcal adsorption of IgG.近期单纯疱疹感染的诊断。一种经改良的免疫荧光试验，用于在葡萄球菌吸附IgG后检测特异性单纯疱疹IgM抗体。
Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Jun;82(3):323-8.
4
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6
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