Walzem R L, Clifford C K, Clifford A J
J Nutr. 1983 Feb;113(2):421-9. doi: 10.1093/jn/113.2.421.
Growth rate, hematological changes, serum, erythrocyte and liver folate levels, and urinary excretion of formiminoglutamic acid (FIGLU) were measured in rats fed p-aminobenzoic acid (PABA)-free, sulfonamide-supplemented purified amino acid diets with and without folic acid and fiber, individually and in combination. Fiber had no effect on growth but was necessary to prevent mortality in the absence of folic acid. Folic acid did not affect growth during the first 40 days, but after this period rats failed to gain weight in the absence of folic acid. Although blood hematocrit was lower when the diet was devoid of fiber and folic acid and leucocyte counts were lower when the diets were devoid of folic acid, the hematological values remained within normal physiological limits for healthy rats of comparable age. FIGLU excretion was increased and serum, erythrocyte and liver folate levels were depressed when rats were fed folic acid-free diets for 28 days. Neutrophil hypersegmentation was clearly evident after 28 days of feeding the folic acid-free diets. The use of an amino acid diet without PABA but containing 5% fiber and 1% succinylsulfathiazole is a useful system to study chronic folic acid deficiency in the rat.
在单独或组合给予无对氨基苯甲酸（PABA）、补充了磺胺类药物的纯化氨基酸饮食（含或不含叶酸和纤维）的大鼠中，测定了生长速率、血液学变化、血清、红细胞和肝脏叶酸水平以及亚胺甲基谷氨酸（FIGLU）的尿排泄量。纤维对生长无影响，但在缺乏叶酸时对预防死亡是必需的。叶酸在最初40天内对生长无影响，但在此之后，缺乏叶酸的大鼠体重不再增加。尽管当饮食中缺乏纤维和叶酸时血细胞比容较低，当饮食中缺乏叶酸时白细胞计数较低，但这些血液学值仍在同龄健康大鼠的正常生理范围内。当大鼠喂食无叶酸饮食28天时，FIGLU排泄增加，血清、红细胞和肝脏叶酸水平降低。喂食无叶酸饮食28天后，中性粒细胞核分叶过多明显可见。使用不含PABA但含有5%纤维和1%琥珀酰磺胺噻唑的氨基酸饮食是研究大鼠慢性叶酸缺乏的有用系统。
