Toowicharanont P, Chulavatnatol M
J Reprod Fertil. 1983 Jan;67(1):133-41. doi: 10.1530/jrf.0.0670133.
Sialoglycoproteins of rat epididymal fluid and spermatozoa were radiolabelled by the NaIO4/KB3H4 method. At least 10 sialoglycoproteins of the epididymal fluids could be consistently demonstrated by polyacrylamide gel electrophoresis in sodium dodecyl sulphate. Two major ones (Mr 21000 and 66000) were present in the fluids of the caput and cauda epididymidis. Two (Mr 28000 and 40000) were found only in the former and two (Mr 32000 and 42000) only in the latter. There were at least 11 sialoglycoproteins bound to the epididymal spermatozoa. During epididymal transport, 8 sialoglycoproteins on the spermatozoa decreased, one (Mr 48000) remained constant and one (Mr 31000) increased in amount. During sperm maturation, some sperm-bound sialoglycoproteins, especially 3 of small molecular weights, became more resistant to the treatments with neuraminidase, trypsin and Triton X-100.
大鼠附睾液和精子的唾液酸糖蛋白通过高碘酸钠/硼氢化钾法进行放射性标记。通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳可始终显示出至少10种附睾液中的唾液酸糖蛋白。附睾头和附睾尾的液体中存在两种主要的唾液酸糖蛋白（分子量分别为21000和66000）。两种（分子量28000和40000）仅存在于附睾头的液体中，两种（分子量32000和42000）仅存在于附睾尾的液体中。至少有11种唾液酸糖蛋白与附睾精子结合。在附睾运输过程中，精子上的8种唾液酸糖蛋白减少，一种（分子量48000）保持不变，一种（分子量31000）数量增加。在精子成熟过程中，一些与精子结合的唾液酸糖蛋白，尤其是3种小分子的唾液酸糖蛋白，对神经氨酸酶、胰蛋白酶和曲拉通X-100的处理更具抗性。
