Toowicharanont P, Chulavatnatol M
J Reprod Fertil. 1983 Mar;67(2):275-80. doi: 10.1530/jrf.0.0670275.
Bound sialic acids on rat spermatozoa were assayed by oxidation with 1 mM-NaIO4 at 0 degree C, liberating C-9 as formaldehyde which was further quantitated using 3-methyl-2-benzothiazolinone. The mean +/- s.d. (n = 20) content of bound sialic acids of spermatozoa from the caput and cauda epididymidis was 50.9 +/- 8.0 and 25.2 +/- 3.8 nmol/10(8) spermatozoa respectively. About 85% of the former and 75% of the latter could be extracted by 1% Triton X-100 and 2 mM-dithiothreitol. About 70% of the former and 20% of the latter were released by neuraminidase from Vibrio cholerae. About 40% of the former and 30% of the latter were sensitive to trypsin. During sperm maturation, the decrease in the total bound sialic acids was due to the decrease in the neuraminidase-sensitive but not the neuraminidase-resistant sialic acids.
在0℃下用1 mM高碘酸钠氧化法测定大鼠精子上结合的唾液酸，释放出的C-9以甲醛形式存在，再用3-甲基-2-苯并噻唑啉酮进一步定量。来自附睾头和附睾尾的精子结合唾液酸的平均含量±标准差（n = 20）分别为50.9±8.0和25.2±3.8 nmol/10⁸精子。前者约85%和后者约75%可被1% Triton X-100和2 mM二硫苏糖醇提取。前者约70%和后者约20%可被霍乱弧菌神经氨酸酶释放。前者约40%和后者约30%对胰蛋白酶敏感。在精子成熟过程中，结合唾液酸总量的减少是由于神经氨酸酶敏感的而非神经氨酸酶抗性的唾液酸减少。
