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携带复制不相容区域和插入元件IS1的R12衍生质粒中RNA聚合酶结合位点的定位。

Mapping of RNA polymerase binding sites in R12 derived plasmids carrying the replication-incompatibility region and the insertion element IS1.

作者信息

Chan P T, Lebowitz J

出版信息

Nucleic Acids Res. 1982 Nov 25;10(22):7295-311. doi: 10.1093/nar/10.22.7295.

DOI:10.1093/nar/10.22.7295
PMID:6296771
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327005/
Abstract

Interactions between Escherichia coli RNA polymerase holoenzyme and three small plasmid DNAs (pSM1, pSM2, and pSM15) derived from the drug resistant factor R12 have been studied. These plasmids carry the copy number and incompatibility determinants, the origin of DNA replication and the rep gene(s) necessary for plasmid replication. They also contain the insertion element IS1 and the putative finO cistron. Thirteen DNA segments within the largest of the three plasmids (pSM2) were able to form either a binary and/or ternary complex with RNA polymerase. A unique strong binding site was mapped within the left end of IS1. Five binding sites were found within the rep-cop-inc region. Four of these are weak binding sites whereas the fifth does not form a stable binary complex and was detected by ternary complex formation. A strong binding site was located in the putative finO region whereas the remaining six binding sites are located in regions with unidentified genetic functions.

摘要

对大肠杆菌RNA聚合酶全酶与源自耐药因子R12的三种小质粒DNA（pSM1、pSM2和pSM15）之间的相互作用进行了研究。这些质粒携带拷贝数和不相容性决定簇、DNA复制起点以及质粒复制所需的rep基因。它们还包含插入元件IS1和推定的finO顺反子。三种质粒中最大的质粒（pSM2）内的13个DNA片段能够与RNA聚合酶形成二元和/或三元复合物。在IS1的左端定位了一个独特的强结合位点。在rep-cop-inc区域内发现了五个结合位点。其中四个是弱结合位点，而第五个不形成稳定的二元复合物，通过三元复合物形成检测到。一个强结合位点位于推定的finO区域，而其余六个结合位点位于遗传功能不明的区域。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca1d/327005/bf0b06744e8d/nar00391-0249-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca1d/327005/b8775feca116/nar00391-0245-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca1d/327005/3182a861c4c4/nar00391-0247-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca1d/327005/08f7295e2a52/nar00391-0247-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca1d/327005/92d4e4fc500a/nar00391-0249-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca1d/327005/bf0b06744e8d/nar00391-0249-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca1d/327005/b8775feca116/nar00391-0245-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca1d/327005/3182a861c4c4/nar00391-0247-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca1d/327005/08f7295e2a52/nar00391-0247-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca1d/327005/92d4e4fc500a/nar00391-0249-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca1d/327005/bf0b06744e8d/nar00391-0249-b.jpg

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本文引用的文献

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2
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Mol Gen Genet. 1981;183(3):490-6. doi: 10.1007/BF00268770.
3
Genes and sites involved in replication and incompatibility of an R100 plasmid derivative based on nucleotide sequence analysis.
促进大肠杆菌K12插入元件IS30上的RNA转录。
EMBO J. 1985 Oct;4(10):2687-93. doi: 10.1002/j.1460-2075.1985.tb03988.x.
4
Translational control of transposition activity of the bacterial insertion sequence IS1.细菌插入序列IS1转座活性的翻译调控
EMBO J. 1991 Mar;10(3):705-12. doi: 10.1002/j.1460-2075.1991.tb08000.x.
基于核苷酸序列分析的R100质粒衍生物复制与不相容性相关的基因和位点
Mol Gen Genet. 1980;179(3):527-37. doi: 10.1007/BF00271742.
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