人类β2 - 肾上腺素能受体基因的染色体定位
Chromosomal assignment of the gene for the human beta 2-adrenergic receptor.
作者信息
Sheppard J R, Wehner J M, McSwigan J D, Shows T B
出版信息
Proc Natl Acad Sci U S A. 1983 Jan;80(1):233-6. doi: 10.1073/pnas.80.1.233.
Chinese hamster fibroblasts (CHW) fail to physiologically respond to the beta-adrenergic agonist isoproterenol with an increase in cellular cAMP. This unresponsiveness is due to a lack of beta-adrenergic receptors as indicated by an absence of specific binding of 125I-labeled hydroxybenzylpindolol (125I-HYP) to CHW plasma membranes. Preparation of somatic cell hybrids between CHW and human peripheral blood leukocytes led to the selection of a panel of 15 human-Chinese hamster cell hybrid clones, some of which had beta 2-adrenergic receptors (specifically bound 125I-HYP) and responded to isoproterenol (accumulated cAMP). Biochemical analysis of independent cloned cell hybrids indicated that beta 2-adrenergic receptor density and the intensity of the associated physiological response were closely correlated (r = 0.98). Both of these parameters were concordant in all cell hybrids with the presence of human chromosome 5. All other human chromosomes could be ruled out. These results suggest that the structural gene for the beta 2-adrenergic receptor is found on human chromosome 5.
中国仓鼠成纤维细胞（CHW）对β-肾上腺素能激动剂异丙肾上腺素不能产生细胞内环磷酸腺苷（cAMP）增加的生理反应。这种无反应性是由于缺乏β-肾上腺素能受体，这一点可通过125I标记的羟基苄基吲哚洛尔（125I-HYP）与CHW质膜缺乏特异性结合来表明。制备CHW与人外周血白细胞之间的体细胞杂种，导致选择了一组15个人-中国仓鼠细胞杂种克隆，其中一些具有β2-肾上腺素能受体（特异性结合125I-HYP）并对异丙肾上腺素产生反应（积累cAMP）。对独立克隆的细胞杂种进行生化分析表明，β2-肾上腺素能受体密度与相关生理反应强度密切相关（r = 0.98）。在所有具有人类5号染色体的细胞杂种中，这两个参数都是一致的。所有其他人类染色体均可排除。这些结果表明，β2-肾上腺素能受体的结构基因位于人类5号染色体上。
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