Kobilka B K, Dixon R A, Frielle T, Dohlman H G, Bolanowski M A, Sigal I S, Yang-Feng T L, Francke U, Caron M G, Lefkowitz R J
Proc Natl Acad Sci U S A. 1987 Jan;84(1):46-50. doi: 10.1073/pnas.84.1.46.
We have isolated and sequenced a cDNA encoding the human beta 2-adrenergic receptor. The deduced amino acid sequence (413 residues) is that of a protein containing seven clusters of hydrophobic amino acids suggestive of membrane-spanning domains. While the protein is 87% identical overall with the previously cloned hamster beta 2-adrenergic receptor, the most highly conserved regions are the putative transmembrane helices (95% identical) and cytoplasmic loops (93% identical), suggesting that these regions of the molecule harbor important functional domains. Several of the transmembrane helices also share lesser degrees of identity with comparable regions of select members of the opsin family of visual pigments. We have localized the gene for the beta 2-adrenergic receptor to q31-q32 on chromosome 5. This is the same position recently determined for the gene encoding the receptor for platelet-derived growth factor and is adjacent to that for the FMS protooncogene, which encodes the receptor for the macrophage colony-stimulating factor.
我们已经分离并测序了编码人β2 - 肾上腺素能受体的cDNA。推导的氨基酸序列（413个残基）是一种含有七簇疏水氨基酸的蛋白质序列，这些疏水氨基酸簇提示存在跨膜结构域。虽然该蛋白质与先前克隆的仓鼠β2 - 肾上腺素能受体总体上有87%的同一性，但最保守的区域是推定的跨膜螺旋（95%相同）和胞质环（93%相同），这表明分子的这些区域含有重要的功能结构域。几个跨膜螺旋与视觉色素视蛋白家族特定成员的可比区域也有较低程度的同一性。我们已经将β2 - 肾上腺素能受体基因定位到5号染色体的q31 - q32。这与最近确定的血小板衍生生长因子受体编码基因的位置相同，并且与FMS原癌基因相邻，FMS原癌基因编码巨噬细胞集落刺激因子受体。
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