通过免疫沉淀试验直接证实的格雷夫斯病中的抗促甲状腺素受体抗体。

Anti-thyrotrophin receptor antibodies in Graves' disease as demonstrated directly by immunoprecipitation assay.

作者信息

de Bruin T W, van der Heide D

出版信息

Acta Endocrinol (Copenh). 1983 Jan;102(1):49-56. doi: 10.1530/acta.0.1020049.

DOI:10.1530/acta.0.1020049

Abstract

An immunoprecipitation assay was developed to determine the presence of antibodies against human TSH receptors. With this assay we were able to demonstrate that in comparison with sera from normal controls. 24 out of 30 (80%) sera from patients with untreated Graves' disease could immunoprecipitate more [125I]TSH-TSH receptor complexes. In 9 assays, an average of 14.1 +/- 3.7% (SD) of the [125I]TSH-TSH receptor complexes was immunoprecipitated by the 30 Graves' sera vs 9.8 +/- 3.0% by the normal pool serum (n = 23) (P less than 0.001) and 7.7 +/- 2.8% by the 22 normal sera (P less than 0.001). One serum of the 24 positive Graves' sera was studied in detail. The results suggest that this serum contained an anti-TSH receptor auto-antibody directed towards a different determinant on the TSH receptor than the TSH binding site.

摘要

开发了一种免疫沉淀试验来确定抗人促甲状腺激素（TSH）受体抗体的存在。通过该试验，我们能够证明，与正常对照血清相比，30例未经治疗的格雷夫斯病患者血清中有24例（80%）能够免疫沉淀更多的[125I]TSH-TSH受体复合物。在9次试验中，30份格雷夫斯病血清平均免疫沉淀了[125I]TSH-TSH受体复合物的14.1±3.7%（标准差），而正常混合血清（n = 23）为9.8±3.0%（P<0.001），22份正常血清为7.7±2.8%（P<0.001）。对24份阳性格雷夫斯病血清中的一份血清进行了详细研究。结果表明，该血清中含有的抗TSH受体自身抗体所针对的TSH受体决定簇与TSH结合位点不同。