Burger D, Sriranganathan N, McDonald T L, Gorham J R
Am J Vet Res. 1983 Jan;44(1):86-90.
Affinity chromatography on immobilized equine complement Clq was used for the isolation of complement-binding immune complexes in sera of mink infected with Aleutian disease virus. Immune complexes were isolated and quantitated from 4 of 5 infected mink, as early as 2 weeks after infection and before hypergammaglobulinemia had appeared. The quantity of immunoglobulin G in these immune complexes ranged from 180 to 370 micrograms/ml serum. There were no Clq-binding immune complexes found in mink which were negative for Aleutian disease antibody. Using 125I-labeled BSA-anti-BSA complexes, we demonstrated that the affinity columns bound selectively immune complexes which had formed in antibody excess, whereas immune complexes in antigen excess were not bound. By neutralization of sensitized virus with anti-mink IgG serum, non Clq-binding immune complexes were also detected, which indicates that circulating immune complexes in persistently infected mink are heterogeneous as far as their reactivity with equine Clq is concerned.
利用固定化马补体C1q进行亲和层析，从感染阿留申病病毒的水貂血清中分离补体结合免疫复合物。早在感染后2周且高球蛋白血症出现之前，就从5只感染水貂中的4只分离并定量了免疫复合物。这些免疫复合物中免疫球蛋白G的含量为每毫升血清180至370微克。在阿留申病抗体呈阴性的水貂中未发现C1q结合免疫复合物。使用125I标记的牛血清白蛋白-抗牛血清白蛋白复合物，我们证明亲和柱选择性结合在抗体过量时形成的免疫复合物，而抗原过量时的免疫复合物则不被结合。通过用抗水貂IgG血清中和致敏病毒，还检测到非C1q结合免疫复合物，这表明就其与马C1q的反应性而言，持续感染水貂中的循环免疫复合物是异质性的。
