Crawford T B, McGuire T C, Porter D D, Cho H J
J Immunol. 1977 Apr;118(4):1249-51.
Four methods of detecting and quantitating mink antibody against Aleutian disease (AD) virus were compared. Counterelectrophoresis, modified, counterelectrophoresis, immunofluorescence, and complement fixation were performed blindly on 274 serum samples. All four methods were reliably specific for AD antibody. Immunofluorescence was less reproducible than the other systems. Immunofluorescence complement fixation were 4- to 8-fold more sensitive than regular or modified counterelectrophoresis, but were limited by background staining and anti-complementary activity, respectively, when used to detect small amounts of antibody in undiluted sera.
对检测和定量水貂抗阿留申病（AD）病毒抗体的四种方法进行了比较。对274份血清样本盲目进行了对流免疫电泳、改良对流免疫电泳、免疫荧光和补体结合试验。所有四种方法对AD抗体均具有可靠的特异性。免疫荧光的重复性不如其他方法。免疫荧光和补体结合试验比常规或改良对流免疫电泳灵敏4至8倍，但在用于检测未稀释血清中的少量抗体时，分别受到背景染色和抗补体活性的限制。
