Tomera J F, Goetz P G, Rand W M, Brunengraber H
Biochem J. 1982 Oct 15;208(1):231-4. doi: 10.1042/bj2080231.
(14)CO(2) production by perfused rat livers was simulated by infusing NaH(14)CO(3) into the perfusate. Recovery of label as (14)CO(2) gas + perfusate bicarbonate was 45-85%. Rates of (14)CO(2) exchange in the liver are 3-70 times greater than net rates of CO(2) production. Therefore (14)CO(2) reincorporation can lead to significant underestimations of rates of oxidation of (14)C-labelled substrates in liver.
(14)通过向灌注液中注入NaH(14)CO(3)来模拟灌注大鼠肝脏产生CO(2)的过程。作为(14)CO(2)气体+灌注液碳酸氢盐的标记物回收率为45 - 85%。肝脏中(14)CO(2)的交换速率比CO(2)产生的净速率大3 - 70倍。因此,(14)CO(2)的再掺入可能导致对肝脏中(14)C标记底物氧化速率的显著低估。