Groves M L, Greenberg R
Biochem J. 1978 Feb 1;169(2):337-42. doi: 10.1042/bj1690337.
The major component of the casein fraction of human milk was cleaved by cyanogen bromide, and the composition of the resulting peptides was determined. Casein was also subjected to limited digestion by trypsin, and the amino acid composition of the isolated peptides was established. With this information the peptides were ordered as they occur in the purified protein.
人乳酪蛋白组分的主要成分用溴化氰裂解,然后测定所得肽段的组成。酪蛋白还用胰蛋白酶进行了有限度的消化,并确定了分离出的肽段的氨基酸组成。利用这些信息,将肽段按其在纯化蛋白质中的出现顺序排列。