吡哆胺-丙酮酸转氨酶中的吡哆醛结合位点。

The pyridoxal-binding site in pyridoxamine-pyruvate transaminase.

作者信息

Hodsdon J, Kolb H, Snell E E, Cole R D

出版信息

Biochem J. 1978 Feb 1;169(2):429-32. doi: 10.1042/bj1690429.

DOI:10.1042/bj1690429

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1184184/

Abstract

The enzyme-substrate complex formed between pyridoxamine-pyruvate transaminase (EC 2.6.1.30) and pyridoxal was reduced with NaBH4. After carboxymethylation and tryptic digestion, pyridoxyl-lysine-containing peptides were isolated by a combination of Sephadex and Dowex 50 chromatography. Analysis of these peptides shows the structure around the pyridoxal-binding lysine residues to be Ala-Asp-Ile-Tyr-Val-Thr-Gly-Pro-Asx-Lys(Pxy)-Cys-Leu(Pro2, Gly2, Ala2, Met)(Thr, Leu2)Gly-Val-Ser-Glu-Arg. This structure differs from those found for the corresponding peptides from pyridoxal phosphate-dependent enzymes.

摘要

用硼氢化钠还原吡哆胺 - 丙酮酸转氨酶（EC 2.6.1.30）与吡哆醛形成的酶 - 底物复合物。经羧甲基化和胰蛋白酶消化后，通过葡聚糖凝胶和Dowex 50色谱相结合的方法分离出含吡哆基赖氨酸的肽段。对这些肽段的分析表明，吡哆醛结合赖氨酸残基周围的结构为Ala - Asp - Ile - Tyr - Val - Thr - Gly - Pro - Asx - Lys(Pxy) - Cys - Leu(Pro2, Gly2, Ala2, Met)(Thr, Leu2)Gly - Val - Ser - Glu - Arg。该结构与磷酸吡哆醛依赖性酶相应肽段的结构不同。

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