• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

猪心丙氨酸转氨酶的磷酸吡哆醛结合位点

Pyridoxal 5'-phosphate binding site of pig heart alanine aminotransferase.

作者信息

Tanase S, Kojima H, Morino Y

出版信息

Biochemistry. 1979 Jul 10;18(14):3002-7. doi: 10.1021/bi00581a015.

DOI:10.1021/bi00581a015
PMID:465450
Abstract

After borohydride reduction, carboxymethylation, and tryptic digestion of the holoenzyme of pig heart alanine aminotransferase, a single icosapeptide containing the N6-(phosphopyridoxyl)lysine residue was isolated by a combination of gel filtration and ion-exchange chromatogrpahy. Its primary structure was determined as Gln-Glu-Leu-Ala-Ser-Phe-His-Ser-Val-Ser-Lsy(Pxy)-Gly-Phe-Met-Gly-Glu-Cys-Gly-Phe-Arg.

摘要

对猪心丙氨酸转氨酶全酶进行硼氢化物还原、羧甲基化和胰蛋白酶消化后，通过凝胶过滤和离子交换色谱法相结合，分离出一种含有N6-(磷酸吡哆醛)赖氨酸残基的单一二十肽。其一级结构确定为Gln-Glu-Leu-Ala-Ser-Phe-His-Ser-Val-Ser-Lsy(Pxy)-Gly-Phe-Met-Gly-Glu-Cys-Gly-Phe-Arg。

相似文献

1
Pyridoxal 5'-phosphate binding site of pig heart alanine aminotransferase.猪心丙氨酸转氨酶的磷酸吡哆醛结合位点
Biochemistry. 1979 Jul 10;18(14):3002-7. doi: 10.1021/bi00581a015.
2
Primary structure of murine major histocompatibility complex alloantigens: amino acid sequence of the amino-terminal one hundred and seventy-three residues of the H-2Kb glycoprotein.小鼠主要组织相容性复合体同种异体抗原的一级结构：H-2Kb糖蛋白氨基末端173个残基的氨基酸序列。
Biochemistry. 1980 Jan 22;19(2):306-15. doi: 10.1021/bi00543a009.
3
Amino acid sequence around the pyridoxal 5'-phosphate binding sites of 6-phosphogluconate dehydrogenase.6-磷酸葡萄糖酸脱氢酶的磷酸吡哆醛5'-磷酸结合位点周围的氨基酸序列。
Biochim Biophys Acta. 1981 Jan 15;657(1):232-42. doi: 10.1016/0005-2744(81)90147-9.
4
Sequence studies on D-serine dehydratase of Escherichia coli. Primary structure of the tryptic phosphopyridoxyl peptide and of the N-terminus.大肠杆菌D-丝氨酸脱水酶的序列研究。胰蛋白酶磷酸吡哆醛肽和N端的一级结构。
Eur J Biochem. 1976 Dec;71(1):109-16. doi: 10.1111/j.1432-1033.1976.tb11095.x.
5
Amino acid sequence of a ferredoxin from thermoacidophilic archaebacterium, Sulfolobus acidocaldarius. Presence of an N6-monomethyllysine and phyletic consideration of archaebacteria.嗜热嗜酸古细菌酸热硫化叶菌铁氧化还原蛋白的氨基酸序列。N6-单甲基赖氨酸的存在及古细菌的系统发育分析
J Biochem. 1985 Mar;97(3):745-53. doi: 10.1093/oxfordjournals.jbchem.a135114.
6
The pyridoxal-binding site in pyridoxamine-pyruvate transaminase.吡哆胺-丙酮酸转氨酶中的吡哆醛结合位点。
Biochem J. 1978 Feb 1;169(2):429-32. doi: 10.1042/bj1690429.
7
Reexamination of the binding site for pyridoxal 5'-phosphate in ribulosebisphosphate carboxylase/oxygenase from Rhodospirillum rubrum.对来自红螺菌的核酮糖二磷酸羧化酶/加氧酶中磷酸吡哆醛结合位点的重新研究。
Biochemistry. 1982 Mar 16;21(6):1380-5. doi: 10.1021/bi00535a043.
8
Primary structure of murine major histocompatibility alloantigens: amino acid sequence of the cyanogen bromide fragment Ia (positions 139-228) from the H-2Kb molecule.小鼠主要组织相容性同种异体抗原的一级结构：来自H-2Kb分子的溴化氰片段Ia（第139 - 228位）的氨基酸序列。
Biochemistry. 1980 Dec 23;19(26):6182-8. doi: 10.1021/bi00567a036.
9
Complete amino acid sequence of ferredoxin from Peridinium bipes (Dinophyceae).双足裸甲藻（甲藻门）铁氧化还原蛋白的完整氨基酸序列
J Biochem. 1988 Nov;104(5):700-5. doi: 10.1093/oxfordjournals.jbchem.a122536.
10
Amino acid sequence of human tumor derived angiogenin.人类肿瘤衍生血管生成素的氨基酸序列。
Biochemistry. 1985 Sep 24;24(20):5486-94. doi: 10.1021/bi00341a031.

引用本文的文献

1
Molecular cloning and differential expression of an gamma-aminobutyrate transaminase gene, OsGABA-T, in rice (Oryza sativa) leaves infected with blast fungus.水稻（Oryza sativa）感染稻瘟病菌叶片中γ-氨基丁酸转氨酶基因OsGABA-T的分子克隆与差异表达
J Plant Res. 2006 Nov;119(6):663-9. doi: 10.1007/s10265-006-0018-3. Epub 2006 Aug 8.
2
Identification and expression of a cDNA clone encoding aspartate aminotransferase in carrot.鉴定和表达胡萝卜天冬氨酸氨基转移酶 cDNA 克隆。
Plant Physiol. 1992 Sep;100(1):374-81. doi: 10.1104/pp.100.1.374.
3
Molecular characterization of a gene for alanine aminotransferase from rice (Oryza sativa).
Plant Mol Biol. 1999 Jan;39(1):149-59. doi: 10.1023/a:1006156214716.
4
Characterization of two glutamate decarboxylase cDNA clones from Arabidopsis.来自拟南芥的两个谷氨酸脱羧酶cDNA克隆的特征分析。
Plant Physiol. 1998 Aug;117(4):1411-21. doi: 10.1104/pp.117.4.1411.
5
Molecular analysis of the yeast SER1 gene encoding 3-phosphoserine aminotransferase: regulation by general control and serine repression.编码3-磷酸丝氨酸氨基转移酶的酵母SER1基因的分子分析：受一般调控和丝氨酸阻遏作用的调节
Curr Genet. 1995 May;27(6):501-8. doi: 10.1007/BF00314439.
6
Three-dimensional structure of a pyridoxal-phosphate-dependent enzyme, mitochondrial aspartate aminotransferase.一种磷酸吡哆醛依赖性酶——线粒体天冬氨酸氨基转移酶的三维结构
Proc Natl Acad Sci U S A. 1980 May;77(5):2559-63. doi: 10.1073/pnas.77.5.2559.
7
Molar absorptivity and A 1% 1cm values for proteins at selected wavelengths of the visible and ultraviolet regions. XXIII.蛋白质在可见光和紫外光区域选定波长下的摩尔吸光系数和百分吸光系数值。二十三。
Appl Biochem Biotechnol. 1984 Apr;9(2):187-206. doi: 10.1007/BF02798752.
8
A common origin for enzymes involved in the terminal step of the threonine and tryptophan biosynthetic pathways.苏氨酸和色氨酸生物合成途径末端步骤中所涉及的酶具有共同起源。
Proc Natl Acad Sci U S A. 1987 Aug;84(15):5207-10. doi: 10.1073/pnas.84.15.5207.
9
Sequence and structure of the dopa decarboxylase gene of Drosophila: evidence for novel RNA splicing variants.果蝇多巴脱羧酶基因的序列与结构：新型RNA剪接变体的证据
EMBO J. 1986 Oct;5(10):2663-72. doi: 10.1002/j.1460-2075.1986.tb04549.x.
10
Characterization and expression of the complementary DNA encoding rat histidine decarboxylase.编码大鼠组氨酸脱羧酶的互补DNA的表征与表达
Proc Natl Acad Sci U S A. 1990 Jan;87(2):733-7. doi: 10.1073/pnas.87.2.733.