通过持续输注测量豚鼠心肌肌球蛋白的合成。
Synthesis of guinea-pig cardiac myosin as measured by constant infusion.
作者信息
Wyborny L E, Kritcher E M, Luchi R J
出版信息
Biochem J. 1978 Jan 15;170(1):189-92. doi: 10.1042/bj1700189.
Abstract
An equation was derived from which the turnover time of individual muscle proteins could be calculated from measurements made at a single time interval in individual animals after initiation of constant intravenous infusion of labelled amino acid. The calculation requires only the specific radioactivities of the amino acid in plasma, in the intracellular fluid and in the protein under study. Pool sizes were not required. When the equation was applied to adult guinea-pig cardiac myosin, the average turnover time was 16 +/- 1 days.
摘要
推导出了一个方程式，根据该方程式，在开始对个体动物持续静脉输注标记氨基酸后的单个时间间隔内所做的测量结果，可计算出个体肌肉蛋白的周转时间。该计算仅需要血浆、细胞内液以及所研究蛋白质中氨基酸的比放射性。不需要池大小的数据。当将该方程式应用于成年豚鼠心肌肌球蛋白时，平均周转时间为16±1天。
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引用本文的文献
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Biochem J. 1981 Jan 15;194(1):365-8. doi: 10.1042/bj1940365.
本文引用的文献
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MOLECULAR CHARACTERISTICS OF CANINE CARDIAC MYOSIN.犬心肌肌球蛋白的分子特征
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Turnover rate of muscle protein measured by constant intravenous infusion of 14C-glycine.通过持续静脉输注14C-甘氨酸测量肌肉蛋白的周转率。
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Effect of age on protein and ribonucleic acid metabolism in mouse skeletal muscle.年龄对小鼠骨骼肌蛋白质和核糖核酸代谢的影响。
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6
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Regulation of protein synthesis in heart muscle. I. Effect of amino acid levels on protein synthesis.心肌中蛋白质合成的调节。I. 氨基酸水平对蛋白质合成的影响。
J Biol Chem. 1971 Apr 10;246(7):2152-62.
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Myosin, myoglobin, and collagen synthesis in acute cardiac overload.急性心脏负荷过重时的肌球蛋白、肌红蛋白和胶原蛋白合成
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