血浆区室与心脏中亮氨酰 - tRNA之间亮氨酸的平衡，以及心肌肌球蛋白重链的周转。
Equilibration of leucine between the plasma compartment and leucyl-tRNA in the heart, and turnover of cardiac myosin heavy chain.
作者信息
Everett A W, Prior G, Zak R
出版信息
Biochem J. 1981 Jan 15;194(1):365-8. doi: 10.1042/bj1940365.
Abstract
By 30min continuous infusion of [3H]leucine into rats, the specific radioactivities of plasma leucine and tissue-free and tRNA-bound leucine in heart were equal. The specific radioactivity of leucyl-tRNA in heart therefore follows a time course identical with that of plasma leucine soon after the start of infusion. The half-life of cardiac myosin heavy chain (5.5 days) was the same as that reported by other investigators who used the pulse-labelling protocol.
摘要
通过向大鼠连续输注[³H]亮氨酸30分钟，心脏中血浆亮氨酸、游离组织亮氨酸和tRNA结合亮氨酸的比放射性相等。因此，心脏中亮氨酰-tRNA的比放射性在输注开始后不久呈现出与血浆亮氨酸相同的时间进程。心肌肌球蛋白重链的半衰期（5.5天）与其他使用脉冲标记方案的研究者报道的相同。
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