Osterlund M, Luthman H, Nilsson S V, Magnusson G
Gene. 1982 Nov;20(1):121-5. doi: 10.1016/0378-1119(82)90093-2.
We analyzed the effect of ethidium bromide (EtBr) on the cleavage of closed circular pBR322 DNA molecules by six restriction enzymes which make staggered or flush cuts (EcoRI, HindIII, BglI, PstI, HincII, PvuII). EtBr concentrations and reaction temperatures were determined at which DNA molecules with single-strand breaks were the major reaction product of digestion by all the enzymes. However, the amounts of intermediates which could be isolated differed for various enzymes. The results extend previous studies, showing that sequential cleavage of the DNA strands probably is a general property of restriction endonucleases.
我们分析了溴化乙锭（EtBr）对六种限制酶切割闭合环状pBR322 DNA分子的影响，这六种限制酶可产生交错切割或平端切割（EcoRI、HindIII、BglI、PstI、HincII、PvuII）。确定了溴化乙锭浓度和反应温度，在此条件下，单链断裂的DNA分子是所有酶消化的主要反应产物。然而，不同酶可分离出的中间产物量有所不同。这些结果扩展了先前的研究，表明DNA链的顺序切割可能是限制内切酶的普遍特性。
