Warr J L, Branda L A
Gynecol Obstet Invest. 1983;15(2):103-18. doi: 10.1159/000299400.
Phospholipase C (Clostridium welchii and Bacillus cereus) treatment of lactating rabbit mammary gland membranes (140,000 g pellet and sucrose density gradient purified plasma membranes) resulted in a large decrease in the binding of [3H]oxytocin to these subcellular fractions. This decrease was not due to a solubilization of oxytocin receptors but was the result of the removal of phospholipids which may participate in the hormone-receptor interaction. Phospholipase C treatment of the membrane fractions resulted in a dose-dependent removal of different classes of phospholipids. Sphingomyelin, phosphatidylcholine and phosphatidylethanolamine were removed by phospholipase C (C. welchii and B. cereus) treatment. No significant change was observed in the content of phosphatidylinositol. Phospholipase C from B. cereus reduced the content of phosphatidylserine, while the enzyme from C. welchii did not.
用磷脂酶C（韦氏梭菌和蜡样芽孢杆菌）处理泌乳兔乳腺膜（140,000g沉淀和经蔗糖密度梯度纯化的质膜），导致[3H]催产素与这些亚细胞组分的结合大幅减少。这种减少并非由于催产素受体的溶解，而是去除了可能参与激素 - 受体相互作用的磷脂的结果。用磷脂酶C处理膜组分导致不同种类磷脂的剂量依赖性去除。经磷脂酶C（韦氏梭菌和蜡样芽孢杆菌）处理后，鞘磷脂、磷脂酰胆碱和磷脂酰乙醇胺被去除。磷脂酰肌醇含量未观察到显著变化。蜡样芽孢杆菌的磷脂酶C降低了磷脂酰丝氨酸的含量，而韦氏梭菌的酶则没有。
