Shertzer H G
Food Chem Toxicol. 1983 Feb;21(1):31-5. doi: 10.1016/0278-6915(83)90265-x.
Indole-3-carbinol (I-3-C) is a compound present in many cruciferous vegetables that has been shown to reduce aryl hydrocarbon-induced neoplasia in experimental animals. We examined the relationship between the ability of I-3-C to alter the activity of hepatic aryl hydrocarbon hydroxylase (AHH), and its ability to inhibit the covalent binding of benzo[a]pyrene (BaP) metabolites to DNA and protein. Using an in vitro system and a hepatic postmitochondrial fraction from mice that had been treated by gavage with I-3-C, we found that up to 90% of the covalent binding of BaP metabolites to macromolecules was eliminated, while AHH activity was unchanged. In experiments in vivo, treatment of mice by gavage with I-3-C before [14C]BaP resulted in up to an 80% decrease in covalent binding of 14C to DNA or protein with no concomitant decrease in hepatic AHH activity. These results suggest that I-3-C administered in vivo confers protection against the binding of BaP oxidation products to hepatic cellular macromolecules.
吲哚 - 3 - 甲醇（I - 3 - C）是一种存在于多种十字花科蔬菜中的化合物，已证明其能在实验动物中减少芳烃诱导的肿瘤形成。我们研究了I - 3 - C改变肝脏芳烃羟化酶（AHH）活性的能力与其抑制苯并[a]芘（BaP）代谢产物与DNA和蛋白质共价结合能力之间的关系。使用体外系统和经灌胃给予I - 3 - C处理的小鼠肝脏线粒体后组分，我们发现BaP代谢产物与大分子的共价结合消除了高达90%，而AHH活性未改变。在体内实验中，在给予[14C]BaP之前给小鼠灌胃I - 3 - C，导致14C与DNA或蛋白质的共价结合减少高达80%，而肝脏AHH活性并未随之降低。这些结果表明，体内给予I - 3 - C可对BaP氧化产物与肝脏细胞大分子的结合起到保护作用。
