Sivell L M, Wenlock R W, Jackson P A
Hum Nutr Appl Nutr. 1982 Dec;36(6):430-7.
Reverse phase high performance liquid chromatography (HLPC) was used to estimate the vitamin D3 content of battery and free range eggs. At the same time another HPLC technique was used to estimate the retinol activity in the battery eggs derived from the separate determination of all-trans retinol, 13-cis retinol and retinaldehyde concentrations. There was some variation in both the vitamin D3 and retinol activity in eggs over the laying year but no significance could be drawn from this. Vitamin D3 levels in 12 monthly samples each of 120 battery eggs averaged 1.2 micrograms/100 g edible portion, while the levels in each of four quarterly samples of 36 free range eggs from the London and Newcastle areas averaged 1.4 and 0.8 micrograms/100 g respectively. These differences between battery and free range eggs were not statistically significant. The average retinol activity in four samples of 120 battery eggs was calculated to be 190 micrograms/100 g, and the components of this activity were identified as 132 micrograms all-trans retinol and 21 micrograms of retinaldehyde, as well as 53 micrograms 13-cis retinol which has not previously been reported in hens' eggs.
采用反相高效液相色谱法（HLPC）测定笼养蛋和散养蛋中的维生素D3含量。同时，运用另一种高效液相色谱技术，通过分别测定全反式视黄醇、13-顺式视黄醇和视黄醛的浓度来估算笼养蛋中的视黄醇活性。在产蛋年度内，鸡蛋中的维生素D3和视黄醇活性均存在一定差异，但据此无法得出显著结论。120个笼养蛋的12个月度样本中，维生素D3含量平均为1.2微克/100克可食用部分，而来自伦敦和纽卡斯尔地区的36个散养蛋的四个季度样本中，维生素D3含量分别平均为1.4微克/100克和0.8微克/100克。笼养蛋和散养蛋之间的这些差异无统计学意义。120个笼养蛋的四个样本中的平均视黄醇活性经计算为190微克/100克，该活性的成分被鉴定为132微克全反式视黄醇、21微克视黄醛，以及53微克13-顺式视黄醇，13-顺式视黄醇此前在鸡蛋中尚未有过报道。
