Malamed S, Carsia R V
J Gerontol. 1983 Mar;38(2):130-6. doi: 10.1093/geronj/38.2.130.
To study intrinsic age-related changes in adrenocortical steroid production, cells isolated from rats of different ages (3 to 24 months) were used. Acute (2 hour) corticosterone production in response to stimulation by adrenocorticotrophic hormone (ACTH) and adenosine 3':5'-cyclic monophosphate (cAMP) was measured by radioimmunoassay. With age, adrenocortical cells lose much of their ability to produce corticosterone in the absence or presence of ACTH or cAMP. The loss is progressive from 6 to 24 months of age. Analysis of the data suggests that from 6 to 12 months, an intracellular steroidogenic lesion develops; in addition there may be a loss in ACTH receptors on the plasma membrane. After 12 months these defects increase and are accompanied by a decrease in receptor sensitivity to ACTH.
为研究肾上腺皮质类固醇生成过程中与年龄相关的内在变化，使用了从不同年龄（3至24个月）大鼠分离出的细胞。通过放射免疫分析法测定了促肾上腺皮质激素（ACTH）和3':5'-环磷酸腺苷（cAMP）刺激后急性（2小时）皮质酮的生成量。随着年龄增长，无论有无ACTH或cAMP，肾上腺皮质细胞产生皮质酮的能力都会大幅下降。这种下降在6至24个月龄时呈渐进性。数据分析表明，6至12个月时，细胞内类固醇生成发生损伤；此外，质膜上的ACTH受体可能会减少。12个月后，这些缺陷加剧，并伴有受体对ACTH的敏感性降低。
