Houghton J A, Taylor D M
Br J Cancer. 1978 Feb;37(2):199-212. doi: 10.1038/bjc.1978.28.
The effect of serial passage in immune-deprived mice on certain biological and biochemical parameters has been studied in a series of 6 human colorectal tumour xenografts. Histological integrity is maintained for up to 10 serial passages, together with production of epithelial mucins and carcinoembryonic antigen. Passaged tumours retain human lactate dehydrogenase and glucose-6-phosphate dehydrogenase isoenzyme patterns and a human chromosome constitution. The induction of a murine tumour has been identified in this system, and the importance of routine checks for the presence of human tissue during serial passage is stressed.
在一系列6个人类结直肠癌异种移植模型中，研究了在免疫缺陷小鼠中连续传代对某些生物学和生化参数的影响。组织学完整性在连续传代多达10次时得以维持，同时伴有上皮粘蛋白和癌胚抗原的产生。传代后的肿瘤保留了人类乳酸脱氢酶和葡萄糖-6-磷酸脱氢酶同工酶模式以及人类染色体组成。在该系统中已鉴定出诱导产生的鼠源性肿瘤，并强调了在连续传代过程中对人类组织存在情况进行常规检查的重要性。
