Pirisi L, Pascale R, Daino L, Frassetto S, La Spina V, Zanetti S, Gaspa L, Ledda G M, Garcea R, Feo F
Res Commun Chem Pathol Pharmacol. 1982 Nov;38(2):301-11.
Human skin fibroblasts, isolated in vitro, from donors carrying the Mediterranean variant of glucose-6-phosphate dehydrogenase, exhibit a sharp decrease of hexose monophosphate shunt and NADPH/NADP+ ratio when compared to the fibroblasts from normal donors. This behavior is coupled to an increase of the resistance to cell death and growth inhibition induced by benz(a)pyrene, whose activation proceeds through the NADPH-dependent arene oxide formation. No differences were observed in the toxic effects of methylnitrosourea, a carcinogen that does not need metabolic activation, on normal and variant fibroblasts.
从携带葡萄糖-6-磷酸脱氢酶地中海变体的供体体外分离出的人皮肤成纤维细胞，与正常供体的成纤维细胞相比，其磷酸己糖支路和NADPH/NADP+比值急剧下降。这种行为与对苯并(a)芘诱导的细胞死亡和生长抑制的抗性增加有关，苯并(a)芘的活化是通过NADPH依赖性芳烃氧化物的形成进行的。在正常和变体成纤维细胞上，未观察到不需要代谢活化的致癌物甲基亚硝基脲的毒性作用有差异。
