Karelina T V, Ermilova V D, Rukosuev V S, Kazantseva I A
Arkh Patol. 1982;44(12):29-35.
Myoepithelial cells (MC) were identified and the pattern of their distribution in dysplasia and duct carcinoma of the mammary gland was studied using a monospecific antiserum to smooth-muscle myosin of the human uterus, by the indirect Coons' method under luminescent microscope. Samples of the operation material from 10 patients with fibrous-cystic mastopathy and 26 patients with duct carcinoma were examined. In solid (7 observations) and comedo (4 observations) carcinomas with the structure of cancer in situ, MC were found intact in the periphery. In invasive growth of these tumors as well as in scirrhus (5 observations) and adenocarcinoma (10 observations) MC disappeared from the duct structures and were absent in carcinoma proliferates. Positive immunofluorescence in the conventional location of MC in the alveolar duct system of the mammary gland allows sufficiently valid identification and study of the distribution and changes in these cells. The immunocytochemical verification of MC in dysplasia and duct carcinoma indicates the possibility of using MC as markers of duct carcinoma in situ; MC disappearance from the duct structures characterizes the beginning of the infiltrative tumor growth.
采用针对人子宫平滑肌肌球蛋白的单特异性抗血清，通过间接库恩氏法在荧光显微镜下鉴定肌上皮细胞（MC），并研究其在乳腺发育异常和导管癌中的分布模式。对10例纤维囊性乳腺病患者和26例导管癌患者的手术材料样本进行了检查。在具有原位癌结构的实性癌（7例观察）和粉刺癌（4例观察）中，发现MC在周边完整。在这些肿瘤的浸润性生长以及硬癌（5例观察）和腺癌（10例观察）中，MC从导管结构中消失，在癌增殖中不存在。在乳腺腺泡导管系统中MC的常规位置出现的阳性免疫荧光，使得能够充分有效地鉴定和研究这些细胞的分布及变化。在发育异常和导管癌中对MC进行免疫细胞化学验证表明，有可能将MC用作原位导管癌的标志物；MC从导管结构中消失是浸润性肿瘤生长开始的特征。
