Bradbury E, Mithcell W M
Scan Electron Microsc. 1979(3):139-52.
Tissues from seventy-one patients were obtained within one-half hour of biopsy, mastectomy, or reduction mammoplasty and processed for scanning electron microscopy (SEM). Epithelial structures were detected by supravital staining with methylene blue and isolated by microdissection. Mammary lobules and ductules required bacterial collagenase digestion to remove the covering stroma for optimal visualization. Major histopathological categories were compared and correlated with the characteristics observed with methylene blue supravital staining and light microscopy and/or transmission electron microscopy as a function of surface features revealed by SEM. Mammary ducts can be readily distinguished from ductules and lobules by their characteristic surface epithelium. Duct epithelium undergoes a variety of changes in fibrocystic disease (FCD) and in carcinoma. Both apocrine and merocrine secretory activity was observed in the surface epithelium. Proliferation of both epithelial and stromal elements was observed in benign fibroadenomata. Intraductal carcinomas were distinguished by their relative lack of surface microvilli. SEM offers a practical tool in the potential identification of premalignant cells of the human breast epithelium.
从71名患者身上获取的组织在活检、乳房切除术或乳房缩小成形术后半小时内获得，并进行扫描电子显微镜（SEM）处理。通过亚甲蓝活体染色检测上皮结构，并通过显微切割分离。乳腺小叶和小导管需要用细菌胶原酶消化以去除覆盖的基质，以实现最佳可视化。比较主要组织病理学类别，并将其与通过亚甲蓝活体染色、光学显微镜和/或透射电子显微镜观察到的特征相关联，这些特征是扫描电子显微镜揭示的表面特征的函数。乳腺导管可通过其特征性表面上皮与小导管和小叶轻松区分。导管上皮在纤维囊性疾病（FCD）和癌中会发生各种变化。在表面上皮中观察到顶浆分泌和局部分泌活动。在良性纤维腺瘤中观察到上皮和基质成分的增殖。导管内癌的特征是相对缺乏表面微绒毛。扫描电子显微镜为潜在识别人类乳腺上皮的癌前细胞提供了一种实用工具。
