Abeywardena M Y, Charnock J S
Biochim Biophys Acta. 1983 Mar 23;729(1):75-84. doi: 10.1016/0005-2736(83)90457-1.
The role of lipids in the modulation of the ouabain-sensitivity of membrane (Na+ + K+)-ATPase from different species has been studied using a reconstitution procedure which promotes lipid exchange during detergent depletion by Sephadex chromatography. Hybrid reconstitution of delipidated (Na+ + K+)-ATPase preparations from bovine brain into the lipids obtained from crab nerve enzyme preparations significantly reduces the sensitivity of the brain enzyme to inhibition by ouabain. Conversely, reconstitution of crab nerve enzyme into the lipids from bovine brain enzyme preparations increases the sensitivity of the crab enzyme to ouabain inhibition. These opposing effects demonstrate the role of membrane lipids in modulating the enzyme-inhibition relationship in preparations from these different species.
利用一种重构程序研究了脂质在调节不同物种膜（钠+钾）-ATP酶哇巴因敏感性中的作用，该程序在通过葡聚糖凝胶色谱法去除去污剂的过程中促进脂质交换。将牛脑脱脂的（钠+钾）-ATP酶制剂与蟹神经酶制剂获得的脂质进行杂交重构，可显著降低脑酶对哇巴因抑制的敏感性。相反，将蟹神经酶重构到牛脑酶制剂的脂质中，则会增加蟹酶对哇巴因抑制的敏感性。这些相反的作用证明了膜脂质在调节这些不同物种制剂中酶抑制关系的作用。
