Zviagil'skaia R A, Zelenshchikova V A, Burbaev D Sh
Biokhimiia. 1983 Jan;48(1):3-10.
Some specific features of cytosol-mitochondria interactions during the growth of the Endomyces magnusii yeast on sucrose media were investigated. Under the given experimental conditions exogenous (cytoplasmic) NADH is the main source of reducing equivalents. Using low temperature EPR spectroscopy, it was demonstrated that exogenous NADH can effectively induce reverse electron transfer in the respiratory chain of the yeast. Fluorimetric assays suggest that End. magnusii mitochondria can utilize the reducing equivalents formed by reverse electron transfer for the reductive amination of alpha-ketoglutarate to form glutamate. The intramitochondrial localization of aminotransferases was postulated.
研究了大孢内孢霉酵母在蔗糖培养基上生长过程中胞质溶胶-线粒体相互作用的一些特定特征。在给定的实验条件下，外源性（细胞质）NADH是还原当量的主要来源。使用低温电子顺磁共振光谱法表明，外源性NADH可以有效地诱导酵母呼吸链中的逆向电子传递。荧光测定表明，大孢内孢霉线粒体可以利用逆向电子传递形成的还原当量将α-酮戊二酸还原胺化形成谷氨酸。推测了氨基转移酶在线粒体内的定位。
