Zviagil'skaia R A, Korosteleva N L, Kotel'nikova A V
Biokhimiia. 1977 Nov;42(11):1990-6.
The properties of mitochondria from the cells of the "fermentative" variant of End. magnusii were studied. The induced fermentative transformation was brought about by a non-balanced vitamin cultivation. It was shown that the "fermentative" variant of End. magnusii represents an interesting model, in which the energy required for the cell functioning is provided for by a high fermentative activity and a normally functioning respiratory chain. The "fermentative" variant mitochondria were tightly coupled and possessed theoretical efficiency during oxidation of NAD-dependent substrates, which suggested the existence of all the three sites of energy coupling and phosphorylation at the substrate level. A specificity of energy regulation of the End. magnusii "fermentative" variant mitochondria, e. g. tight coupling during oxidation of succinate and lack of tight coupling during oxidation of exogenous NADH, is discussed. The tight coupling during succinate oxidation is confirmed by the observation of reverse electron transfer. Thus, the energy-dependent reduction of NAD during succinate oxidation has been firstly demonstrated for the mitochondria of yeast grown on a fermentable substrate.
研究了巨大内孢霉“发酵型”变种细胞中线粒体的特性。诱导的发酵转化是通过不均衡的维生素培养实现的。结果表明，巨大内孢霉的“发酵型”变种是一个有趣的模型，其中细胞功能所需的能量由高发酵活性和正常运作的呼吸链提供。“发酵型”变种线粒体紧密偶联，在依赖NAD的底物氧化过程中具有理论效率，这表明在底物水平上存在所有三个能量偶联和磷酸化位点。讨论了巨大内孢霉“发酵型”变种线粒体能量调节的特异性，例如琥珀酸氧化过程中的紧密偶联以及外源NADH氧化过程中缺乏紧密偶联。琥珀酸氧化过程中的紧密偶联通过反向电子传递的观察得到证实。因此，首次在以可发酵底物生长的酵母线粒体中证明了琥珀酸氧化过程中NAD的能量依赖性还原。
